猫杯状病毒分离株VP1基因序列与致病性分析

doi:10.11843/j.issn.0366-6964.2022.10.025

Abstract

Abstract: In order to understand VP1 genes and pathogenicity of feline calicivirus (FCV), 13 FCV strains were obtained after isolation and identification. The VP1 genes of these FCV strains were obtained by RT-PCR and sequencing. Then, phylogenetic analyses based on the VP1 sequences were performed with reference strains. Cat pathogenicity experiments were carried out with 2 FCV strains. The results showed that the nucleotide sequence similarity among these 13 FCV VP1 genes was 74.3%-99.8%, and the homology of the nucleotide and amino acid was low with reference strains. The characteristics of variation were consistent with FCV. The analysis of phylogenetic tree showed that the major strains in Shanghai were from northern China, and other strains were from foreign countries. Seven VS-FCV strains were obtained by clinical symptoms, phylogenetic tree of VP1 and VS-FCV characteristic amino acid sites analysis. Cat pathogenicity experiments showed that the strains of FCV-SH202101 and FCV-SH202113 could cause the disease and death of cats, and the incubation period was about 1 to 2 days. The FCV strains would be detected on the 3rd day after inoculation. The durations of FCV in different age groups were different, kittens would be 5 days, and adult cats would be 11 to 18 days. So the FCV-SH202101 strain and FCV-SH202113 strain had the same characteristics with VS-FCV strains in pathogenicity. This study enriched the molecular epidemiological data of FCV in China, provided strong evidence for the screening of VS-FCV strains, and also provided technical support for the research of FCV vaccine.

Key words: feline calicivirus, VP1, homology, phylogenetic tree, pathogenicity

CLC Number:

LIU Jian, BAI Yilan, LI Xin, GUI Yaping, JU Houbin, GONG Guohua, XIA Luming, CHEN Weifeng, ZHU Xiaoying, CHANG Xiaojing, LI Zengqiang, TANG Congsheng, WANG Jian, ZHAO Hongjin. VP1 Sequence and Pathogenicity Analysis of Feline Calicivirus Isolates[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3530-3539.

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VP1 Sequence and Pathogenicity Analysis of Feline Calicivirus Isolates

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