成纤维生长因子4在妊娠早期牛胎盘组织的分布及表达特点

doi:10.11843/j.issn.0366-6964.2019.08.018

Abstract

Abstract: Fibroblast growth factor 4(FGF4) is the first FGF molecule that discovered during the mammalian embryo development, which plays a key role in promoting proliferation of trophoblast cells. Abnormal expression of FGF4 affects development of early embryos and affects the growth and function of mouse placenta as well. Until now, FGF4 is not identified in bovine placenta at early pregnancy. In this study, paraffin sections were prepared and stained with HE, PAS to study the structure in bovine placenta at early pregnancy. In addition, immunofluorescence staining and qRT-PCR were used to study the cell location and expression of FGF4 in bovine placenta at early pregnancy. The results showed that Fgf4 mRNA was detected in amnion, cotyledon and caruncle at every detected age, and the expression of every part varied according to region and gestational age. FGF4 was detected in the cytoplasm of mononuclear trophoblast, trophoblast giant cell, caruncle epithelia, endometrium epithelia, and uterine gland, and was strongly stained in trophoblast giant cell and uterine glandular epithelia. FGF4 and its mRNA were exist in bovine placenta at early pregnancy. With the growth of gestational age, the relative expression of FGF4 mRNA was plunged at first, and then increased in fetal placenta (cotyledon); and in maternal placenta (caruncle), the expression was firstly increased and then decreased.

CLC Number:

S852.16

ZHANG Ruiqi, ZHANG Yingli, JIANG Ming, XU Yue, XU Yongping, QING Suzhu. Expression of Fibroblast Growth Factor 4 in Bovine Placenta at Early Pregnancy[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1685-1693.

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Expression of Fibroblast Growth Factor 4 in Bovine Placenta at Early Pregnancy

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