幼年牦牛不同部位皮肤毛囊的组织学结构及TGF-β2与HIF-1α差异性表达分析

doi:10.11843/j.issn.0366-6964.2021.011.024

Abstract

Abstract: This study was conducted to investigate the histological structure and effects of TGF-β2 and HIF-1α on growth and development of hair follicles in young yaks. Ten healthy young yaks, Skin tissues from their neck, back, breast, abdomen, crus, axilla and scrotum were collected. Paraffin sections were prepared from tissues, stained with HE and Sacpic, and then observed under optical microscope after which representative photomicrographs were taken for analysis. Hair follicles were counted in each part of the skin tissue and categorized as either hairy or less hairy skin. Then qRT-PCR, Western blot, and immunohistochemistry techniques were used to preliminary study TGF-β2 and HIF-1α expression and localization in the hairy or less hairy skin of young yaks. The results showed that hair follicles in the skin of young yaks were distributed in the dermis, often accompanied by sweat glands and sebaceous glands. The structure of hair medulla and inner root sheath was incomplete. Sacpic staining showed the inner root sheath of hair follicles as red, the outer root sheath as pale green, and connective tissue sheath as blue-green. The numbers of hair follicles in the abdominal skin and scrotal skin were (2 085±15)·cm^-2 and (158±15)·cm^-2 respectively. Immunohistochemistry results showed that TGF-β2 and HIF-1α were mainly distributed in the epidermis, outer root sheath of hair follicle, sebaceous glands, and sweat glands. The mRNA and protein levels of TGF-β2 in scrotum and axillary were significantly higher than those in abdomen and back. The mRNA and protein levels of HIF-1α in the abdomen were significantly higher than those in the other three sites. In conclusion, young yaks’ hair follicles were in the catagen phase. The abdomen had the largest number of hair follicles, followed by the back, and the scrotum, the least. The expression levels of TGF-β2 and HIF-1α were significantly varied in different parts of the skin, which provided a theoretical basis for further study on the effects of TGF-β2 and HIF-1α on hair follicle growth and development in yak skin.

Key words: young yak, hairy skin, less hairy skin, TGF-β2, HIF-1α

CLC Number:

S852.16

ZHANG Qian, CUI Yan, YU Sijiu, LIAO Bo, ZOU Shengnan, BAI Xuefeng, Seth Yaw Afedo, ZHAO Pengfei, LONG Min. Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens)[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3224-3233.

References 33

[1]	WIENER G, HAN J L, LONG R J. The yak[M]. 2nd ed. Bangkok:The Regional Office for Asia and the Pacific. Food and Agriculture Organization of the United Nations, 2003.
[2]	PARK K. Role of micronutrients in skin health and function[J]. Biomol Ther (Seoul), 2015, 23(3):207-217.
[3]	沈霞芬, 卿素珠. 兽医组织学与胚胎学[M]. 杨凌:西北农林科技大学出版社, 2009.SHEN X F, QING S Z. Veterinary histology and embryology[M]. Yangling:Northwest A&F University Press, 2009. (in Chinese)
[4]	FINDLAY J D, YANG S H. Capillary distribution in cow skin[J]. Nature, 1948, 161(4104):1012-1013.
[5]	胡倩倩, 杨艳丽, 周先建, 等. 皖东黄牛皮肤组织结构特点观察[J]. 安徽科技学院学报, 2014, 28(5):6-9.HU Q Q, YANG Y L, ZHOU X J, et al. Observation on the skin structure characteristics of Wandong yellow cattle[J]. Journal of Anhui Science and Technology University, 2014, 28(5):6-9. (in Chinese)
[6]	王富金, 李寿万, 崔成都. 延边黄牛皮肤微细结构的观察[J]. 延边农学院学报, 1992(1):28-34.WANG F J, LI S W, CUI C D. Observation on the skin microstructure of Yanbian yellow cattle[J]. Journal of Yanbian Agricultural College, 1992(1):28-34. (in Chinese)
[7]	岳静. 不同年龄牦牛皮肤的组织结构观察[D]. 兰州:甘肃农业大学, 2013.YUE J. Observation the histological structure of skin in different age yak (Bos grunniens)[D]. Lanzhou:Gansu Agricultural University, 2013. (in Chinese)
[8]	于川, 崔燕, 杨雪, 等. 不同年龄牦牛皮肤厚度变化研究[J]. 甘肃农业大学学报, 2017, 52(1):21-25.YU C, CUI Y, YANG X, et al. Thickness changes of skin in different age yak (Bos grunniens)[J]. Journal of Gansu Agricultural University, 2017, 52(1):21-25. (in Chinese).
[9]	YANG X, CUI Y, YUE J, et al. The histological characteristics, age-related thickness change of skin, and expression of the HSPs in the skin during hair cycle in yak (Bos grunniens)[J]. PLoS One, 2017, 12(5):e0176451.
[10]	曹迷霞. 成年牦牛皮肤中附属物、毛细血管和神经的组织学研究[D]. 兰州:甘肃农业大学, 2018.CAO M X. Histological study of appendages, capillaries and nerves in the skin of adult yak[D]. Lanzhou:Gansu Agricultural University, 2018. (in Chinese)
[11]	陈玉红, 狄翠霞, 张倩婧, 等. TGF-β/Smads信号传导通路在肿瘤中的作用[J]. 生理科学进展, 2018, 49(3):187-192.CHEN Y H, DI C X, ZHANG Q J, et al. The role of TGF-β/Smads signaling pathway in tumors[J]. Progress in Physiological Sciences, 2018, 49(3):187-192. (in Chinese)
[12]	PATTERSON G I, PADGETT R W. TGFβ-related pathways:roles in Caenorhabditis elegans development[J]. Trends Genet, 2000, 16(1):27-33.
[13]	HIBINO T, NISHIYAMA T. Role of TGF-β2 in the human hair cycle[J]. J Dermatol Sci, 2004, 35(1):9-18.
[14]	MASSAGUÉ J, CHEN Y G. Controlling TGF-β signaling[J]. Genes Dev, 2000, 14(6):627-644.
[15]	HAWINKELS L J A C, VERSPAGET H W, VAN DER REIJDEN J J, et al. Active TGF-β1 correlates with myofibroblasts and malignancy in the colorectal adenoma-carcinoma sequence[J]. Cancer Sci, 2009, 100(4):663-670.
[16]	SHINBO T, FUSHIDA S, TSUKADA T, et al. Protein-bound polysaccharide K suppresses tumor fibrosis in gastric cancer by inhibiting the TGF-β signaling pathway[J]. Oncol Rep, 2015, 33(2):553-558.
[17]	TSUJI Y, DENDA S, SOMA T, et al. A potential suppressor of TGF-β delays catagen progression in hair follicles[J]. J Investig Dermatol Symp Proc, 2003, 8(1):65-68.
[18]	SONG L L, CUI Y, YU S J, et al. TGF-β and HSP70 profiles during transformation of yak hair follicles from the anagen to catagen stage[J]. J Cell Physiol, 2019, 234(9):15638-15646.
[19]	GOLDBERG M A, DUNNING S P, BUNN H F. Regulation of the erythropoietin gene evidence that the oxygen sensor is a heme protein[J]. Science, 1988, 242(4884):1412-1415.
[20]	SEMENZA G L, WANG G L. A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation[J]. Mol Cell Biol, 1992, 12(12):5447-5454.
[21]	SEMENZA G L. Hypoxia-inducible factors in physiology and medicine[J]. Cell, 2012, 148(3):399-408.
[22]	李勇坚, 张桂英, 肖嵘, 等. HIF-1α与iNOS在银屑病皮损中的表达及其与血管生成的关系[J]. 中南大学学报:医学版, 2010, 35(9):952-957.LI Y J, ZHANG G Y, XIAO R, et al. Expression of iNOS and HIF-1α with angiogenesis in affected skin biopsies from patients with psoriasis[J]. Journal of Central South University:Medical Sciences, 2010, 35(9):952-957. (in Chinese)
[23]	戴叶芹, 范卫新, 吴磊, 等. 低氧诱导因子-1α对毛囊细胞的作用[J]. 中国医学科学院学报, 2007, 29(2):217-221.DAI Y Q, FAN W X, WU L, et al. Effect of hypoxia inducible Factor-lα on cells of hair follicle[J]. Acta Academiae Medicinae Sinicae, 2007, 29(2):217-221. (in Chinese)
[24]	雷焕章, 孙奉先, 徐文彪, 等. 牦牛生殖生理及繁殖特性的观察[J]. 中国畜牧杂志, 1964(7):1-3, 5.LEI H Z, SUN F X, XU W B, et al. Observation on reproductive physiology and characteristics of Yak[J]. Chinese Journal of Animal Science, 1964(7):1-3, 5. (in Chinese)
[25]	MIEUSSET R, FOUDA P J, VAYSSE P, et al. Increase in testicular temperature in case of cryptorchidism in boys[J]. Fertil Steril, 1993, 59(6):1319-1321.
[26]	JAHODA C A B. Cellular and developmental aspects of androgenetic alopecia[J]. Exp Dermatol, 1998, 7(5):235-248.
[27]	PAUS R B, HANDJISKI B, EICHMüLLER S, et al. Chemotherapy-induced alopecia in mice. Induction by cyclophosphamide, inhibition by cyclosporine A, and modulation by dexamethasone[J]. Am J Pathol, 1994, 144(4):719-734.
[28]	GALBRAITH G M P, THIERS B H, VASILY D B, et al. Immunological profiles in alopecia areata[J]. Br J Dermatol, 1984, 110(2):163-170.
[29]	SOMA T, OGO M, SUZUKI J, et al. Analysis of apoptotic cell death in human hair follicles in vivo and in vitro[J]. J Invest Dermatol, 1998, 111(6):948-954.
[30]	叶红, 金咸瑢. 低氧诱导因子-1的结构、功能、调节及其与低氧信号转导的关系[J]. 生理科学进展, 2001, 32(1):62-64.YE H, JIN X R. The structure, function, regulation of HIF-1 and its relationship with hypoxic signal transduction[J]. Progress in Physiological Sciences, 2001, 32(1):62-64. (in Chinese)
[31]	LAUZIER M C, MICHAUD M D, DÉRY M C, et al. HIF-1 activation during tumor progression:implications and consequences[J]. Bull Cancer, 2006, 93(4):349-356.
[32]	SHAH K F, DAVIS M, PHILPOTT M. 208 Investigation into the role of HIF-1A stabilisation in hair follicle metabolism[J]. J Invest Dermatol, 2017, 137(10 Suppl 2):S241.
[33]	任钰昕, 崔燕, 邹胜南, 等. 成年牦牛皮肤中血管与神经的组织学观察及HIF-1α在皮肤中的表达[J]. 畜牧兽医学报, 2021, 52(9):2633-2641.REN Y X, CUI Y, ZOU S N, et al. Histological observation of blood vessels and nerves and expression of HIF-1α in adult yaks skin[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9):2633-2641. (in Chinese)

Histological of Hair Follicles from Different Parts of Skin and Expression of TGF-β2 and HIF-1α in Young Yak (Bos grunniens)

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