低氧诱导因子-1α对牦牛肾小管上皮细胞上皮-间质转化的影响

doi:10.11843/j.issn.0366-6964.2020.03.022

Abstract

Abstract: The aim of this study was to discuss the effect of hypoxia-inducible factor 1α(HIF-1α) on renal tubular epithelial-to-mesenchymal transition (EMT) in yak. First of all, the primary culture and identification of yak renal tubular epithelial cells were conducted. Then the third generation cells were chosen to be treated with drugs (DMOG group) and untreated (control group). The expression changes of EMT related genes were detected at both mRNA and protein levels, and the morphological changes of cells were observed. The results showed that renal epithelial cells could be better obtained by using the collagenase Ⅰ+Ⅱ; Immunofluorescence results showed that CK18 expression was positive, but Vimentin and CD31 were negative; The results of qRT-PCR and Western blot showed that mRNA and protein expression levels of HIF-1α, TGF-β1 and α-SMA in DMOG group were extremely significantly higher than those in control group (P<0.01), while the expression of E-cadherin was significantly lower than that in control group (P<0.05). After drug intervention, the cell morphology changed from paving-stone shape to long shuttle, which was identified as mesenchymal cells. Therefore, this study had successfully established a method for isolation and culture of yak renal tubular epithelial cells, and proved that the up-regulation of HIF-1α expression could promote the renal tubular epithelial-to-mesenchymal transition of yak.

Key words: yak, real tubular epithelial cell, isolation and culture, HIF-1α, epithelial-to-mesenchymal transition

CLC Number:

S852.16

BAI Xuefeng, CUI Yan, YU Sijiu, LI Hui, HE Junfeng, LIAO Bo. Effect of HIF-1α on Renal Tubular Epithelial-to-Mesenchymal Transition in Yak[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 620-629.

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Effect of HIF-1α on Renal Tubular Epithelial-to-Mesenchymal Transition in Yak

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