蛋壳胶护膜性状的遗传特性及其与蛋壳品质性状的关联分析

doi:10.11843/j.issn.0366-6964.2018.04.001

Abstract

Abstract:

In this study, we evaluated cuticle quality and other eggshell quality traits in the Rhode Island Red hens, and we also calculated the genetic and phenotypic correlations between cuticle quality and other eggshells quality traits, which could provide theoretical support for cuticle quality optimization and breeding in the domestic brown/pink egg layer, as well as enhancement of antibacterial properties of egg. We measured cuticle qualities of 3 283 Rhode Island Red hens (120 pedigrees) in Beijing Huadu Yukou Poultry Industry Co. LTD.. Cuticle quality heritability, genetic and phenotypic correlation between cuticle quality and other eggshell quality traits were evaluated by DMU software. The results showed that the value of cuticle quality was (24.24±9.13)%, and its heritability was 0.40 (medium heritability). The values of eggshell color (L^*, a^*, b^*), eggshell strength and eggshell thickness were (61.45±4.91), (17.79±2.34), (30.59±2.50), (3.22±0.75) kg·cm^-2 and (324.85±42.37)μm, respectively. Their heritability were from 0.39 to 0.17, which represented heritability from medium to low. Furthermore, the genetic correlations between cuticle quality and eggshell color (L^*, a^*, b^*) were —0.49, 0.43 and —0.27, respectively. Both eggshell strength and eggshell thickness showed weak correlation with cuticle quality, which were 0.02 and 0.13, respectively. The genetic correlation between eggshell strength and eggshell thickness was 0.72. In conclusion, direct selection of cuticle quality (high heritability) could accelerate genetic progress and improve breeding efficiency. Selection of cuticle quality might deepen eggshell color, since it was related to the L^*, a^* and b^* values. Besides, selection of cuticle quality would not significantly impact eggshell strength and thickness owing to low genetic correlations between them.

CLC Number:

S831.2

LI Wen-bo, CHEN Xia, YUAN Zhong-yang, YU Ying-ying, WU Gui-qin, SHI Feng-ying, YANG Ning, XU Gui-yun, ZHENG Jiang-xia. Genetic Characterization of Cuticle and Its Association with Eggshell Quality Traits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 659-666.

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References

[1] MUÑOZ A, DOMINGUEZ-GASCA N, JIMENEZ-LOPEZ C, et al. Importance of eggshell cuticle composition and maturity for avoiding trans-shell Salmonella contamination in chicken eggs[J]. Food Control, 2015, 55:31-38.
[2] WELLMAN-LABADIE O, PICMAN J, HINCKE M T. Antimicrobial activity of the Anseriform outer eggshell and cuticle[J]. CompBiochem Physiol Part BBiochem Mol Biol, 2008, 149(4):640-649.
[3] HINCKE M, GAUTRON J, RODRIGUEZ-NAVARRO A B, et al. The eggshell:structure and protective function[M]//NYS Y, BAIN M, VAN IMMERSEEL F. Improving the safety and quality of eggs and egg products:Egg chemistry, production and consumption. London:Prentice Hall, Inc., 2011:151-182.
[4] WILSON P W, SUTHER C S, BAIN M M, et al. Understanding avian egg cuticle formation in the oviduct:a study of its origin and deposition[J]. Biol Reprod, 2017, 97(1):39-49.
[5] WHITTOW G C. Sturkie's avian physiology[M]. 5th ed. SanDiego:Academic Press, 2000.
[6] PARSONS A H. Structure of the eggshell[J]. Poult Sci, 1982, 61(10):2013-2021.
[7] MANN K. Proteomic analysis of the chicken egg vitelline membrane[J]. Proteomics, 2008, 8(11):2322-2332.
[8] ROSE-MARTEL M, DU J W, HINCKE M T. Proteomic analysis provides new insight into the chicken eggshell cuticle[J]. J Proteomics, 2012, 75(9):2697-2706.
[9] SAMIULLAH S, ROBERTS J R. The eggshell cuticle of the laying hen[J]. World's Poult Sci J, 2014, 70(4):693-707.
[10] KAGE-NAKADAI E, KOBUNA H, KIMURA M, et al. Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans[J]. PLoS One, 2010, 5(1):e8857.
[11] D'ALBA L, TORRES R, WATERHOUSE GIN, et al. What does the eggshell cuticle do? A functional comparison of avian eggshell cuticles[J].Physiol Biochem Zool, 2017, 90(5):588-599.
[12] DOMINGUEZ-GASCA N, MUÑOZ A, RODRIGUEZ-NAVARRO A B. Quality assessment of chicken eggshell cuticle by infrared spectroscopy and staining techniques:a comparative study[J]. Br Poult Sci, 2017, 58(5):517-522.
[13] BIALKA K L, DEMIRCI A, KNABEL S J, et al. Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs[J]. Poult Sci, 2004, 83(12):2071-2078.
[14] GOLE V C, ROBERTS J R, SEXTON M, et al. Effect of egg washing and correlation between cuticle and egg penetration by various Salmonella strains[J]. Int J Food Microbiol, 2014, 182-183:18-25.
[15] LOCK J L, BOARD R G. Persistence of contamination of hens' egg albumen in vitro with Salmonella serotypes[J]. Epidemiol Infect, 1992, 108(3):389-396.
[16] BOARD R G, HALLS N A. The cuticle-a barrier to liquid and particle penetration of the shell of the hen's egg[J]. Br Poult Sci, 1973, 14(1):69-97.
[17] LELEU S, MESSENS W, DE REU K, et al. Effect of egg washing on the cuticle quality of brown and white table eggs[J]. J Food Prot, 2011, 74(10):1649-1654.
[18] BAIN M M, MCDADE K, BURCHMORE R, et al. Enhancing the egg's natural defence against bacterial penetration by increasing cuticle deposition[J]. Anim Genet, 2013, 44(6):661-668.
[19] CAVERO D, SCHMUTZ M, ICKEN W, et al.Attractive eggshell color as a breeding goal[J]. Lohmann Inform, 2012, 47(2):15-21.
[20] ALI A. The relationship between eggshell colour and egg quality traits in table eggs[J]. Indian J Anim Res, 2014, 48(3):290-294.
[21] 张龙超. 鸡蛋品质性状遗传参数及相关候选基因研究[D]. 北京:中国农业大学, 2007.
ZHANG L C. Study on genetic parameters for and candidate genes associated with egg quality traits in chickens[D]. Beijing:China Agricultural University, 2007. (in Chinese)
[22] 刘文博. 鸡产蛋和蛋品质性状全基因组关联分析研究[D]. 北京:中国农业大学, 2009.
LIU W B. Genome-wide association studies for egg production and quality traits in egg-type chickens[D]. Beijing:China Agricultural University, 2009. (in Chinese)
[23] INGRAM D R, HATTEN Ⅲ L F, HOMAN K D. A study on the relationship between eggshell color and eggshell quality in commercial broiler breeders.[J]. Int J Poult Sci, 2008, 7(7):700-703.
[24] MERTENS K, VAESEN I, LOFFEL J, et al. The transmission color value:A novel egg quality measure for recording shell color used for monitoring the stress and health status of a brown layer flock[J]. Poult Sci, 2010, 89(3):609-617.
[25] MESSENS W, GRIJSPEERDT K, REU K, et al. Eggshell penetration of various types of hens' eggs by Salmonella enterica serovar Enteritidis[J]. J Food Prot, 2007, 70(3):623-628.
[26] HASIAK R J, VADEHRA D V, BAKER R C. Lipid composition of the eggexteriors of the chicken (Gallus gallus)[J]. Comp Biochem Physiol, 1970, 37(3):429-435.
[27] RODRÍGUEZ-NAVARRO A B, DOMÍNGUEZ-GASCA N, MUÑOZ A, et al. Change in the chicken eggshell cuticle with hen age and egg freshness[J]. Poult Sci, 2013, 92(11):3026-3035.
[28] SAMIULLAH S, ROBERTS J R. The location of protoporphyrin in the eggshell of brown-shelled eggs[J]. Poult Sci, 2013, 92(10):2783-2788.
[29] WALKER A W, HUGHES B O. Eggshell colour is affected by laying cage design[J]. BrPoult Sci,1998, 39(5):696-699.
[30] SAMIULLAH, CHOUSALKAR K K, ROBERTS J R, et al. Effects of egg shell quality and washing on Salmonella Infantis penetration[J]. Int J Food Microbiol, 2013, 165(2):77-83.
[31] LIU Y C, CHEN T H, WU Y C, et al. Effects of egg washing and storage temperature on the quality of eggshell cuticle and eggs[J]. Food Chem, 2016, 211:687-693.
[32] DUNN I C, JOSEPH N T, BAIN M, et al. Polymorphisms in eggshell organic matrix genes are associated with eggshell quality measurements in pedigree Rhode Island Red hens[J]. Anim Genet, 2009, 40(1):110-114.

Genetic Characterization of Cuticle and Its Association with Eggshell Quality Traits

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