口蹄疫灭活病毒配合枯草芽胞杆菌鼻腔免疫增强牛呼吸道黏膜免疫应答

doi:10.11843/j.issn.0366-6964.2018.02.021

Abstract

Abstract:

Foot-and-mouth disease virus (FMDV) is the causative agent of contagious and economically devastating diseases that affects cloven-hoofed livestock worldwide. An effective defense barrier against FMD would be established if the respiratory tract transmission for virus was being cut off due to FMDV is mainly transmitted by the respiratory tract. In the current study, health cattle were immunized by inactivated FMDV along with Bacillus subtilis administered through intranasal spray to explore the impact on mucosal immune response (respiratory tract) and systemic immunity in cattle. The results showed that the IgA secreting cells significantly increased in cattle nasal mucosae and pulmonary bronchial (P<0.01) after intranasal immunization with inactivated FMDV along with Bacillus subtilis, but the conventional inactivated FMDV vaccine had no significant effect on the number of IgA secreting cells (P>0.05). At the same time, the level of IL-12 and TNF-α significantly increased in nose, trachea, pulmonary and pulmonary bronchus (P<0.01), especially in the trachea and pulmonary bronchus, but IL-6 level have no significantly difference (P>0.05). O type FMDV specific SIgA from nasal, saliva and O type FMDV specific antibodies in serum significantly increased at 3 days after intranasal immunization, and maintained for 3 months (P<0.01 or P<0.05). These results indicated that both cellular immunity and local humoral immunity of the bovine respiratory tract were enhanced by intranasal immunization with inactivated FMDV along with Bacillus subtilis, as well as the systemic immune response. Our study provided an easier method and prospect for application of whole inactivated virus.

CLC Number:

LI Yi-jiang, ZHU Ming-wang, PU Shi-hua, GUO Yun-ran, YANG Yong-xing, LI Shi-yu, CHUANG Xiang-hui, YANG Qian. By Intranasal Immunization with Inactivated Foot-and-Mouth Disease Virus and Bacillus subtilis Enhance Respiratory Tract Immune Response in Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 412-421.

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By Intranasal Immunization with Inactivated Foot-and-Mouth Disease Virus and Bacillus subtilis Enhance Respiratory Tract Immune Response in Cattle

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