建立及优化肠炎沙门菌的诱导耐酸反应模型

doi:10.11843/j.issn.0366-6964.2018.02.020

Abstract

Abstract:

This study was designed to investigate effects of acid on survival of Salmonella enteritidis. In this study, Box-Behnken design with response surface methodology was used to create the optimal conditions to evaluate the growth of Salmonella under acetic acid pressure environment. The changes of S. enteritidis survivability and the relative expression of acid tolerance-related genes were compared before and after optimization. This model was also used to compare the different relative expression of acid tolerance-related genes in S. enteritidis which induced by other common acids. The results showed that the optimum favorable conditions were as follows:incubation temperature 22.5℃, shaking time of 6 h, amount of bacteria added 15%.Validation experiments showed that bacterial colonies count were 3.966×10⁸ CFU·mL^-1, which basically met with the standard value. At the same time, the survival of S. enteritidis was highest in this environmental condition, and the relative expression of acid tolerance genes were significantly higher (P<0.01). The expressions of acid tolerance genes were also significantly higher (P<0.01) under acidic stress conditions created by other commonly used acids. Our study indicates that the model established by response surface methodology has a reliable tool which could be reasonably used in bacterial acid tolerance induction conditions during optimization.

CLC Number:

S852.61

WANG Yan-qiu, WU Li-yun, CHEN Xiao-gang, SUN Tong, LI Chong, YU Dao-jin. Establishment and Optimization of the Acid Tolerance Response Model for Salmonella Enteritidis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(2): 401-411.

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Establishment and Optimization of the Acid Tolerance Response Model for Salmonella Enteritidis

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