蛋鸡胱硫醚β合酶基因启动子鉴定与分析

doi:10.11843/j.issn.0366-6964.2018.01.024

Abstract

Abstract:

To provide the theoretical basis for exploring the regulatory mechanism of cystathionine beta synthase(CBS) gene expression in layer bone, the 5' regulatory region and core promoter characteristics were investigated. The tibias were collected from laying hen at 68 weeks old. The genomic DNA was extracted with phenol-chloroform method. The primers for amplifying 5' regulatory region of CBS gene were designed according to the template sequence downloaded from UCSC database. The characteristic of 5' regulatory region and prediction of transcription factors binding sites were analyzed by bioinformatics methods. The constructed dual-luciferase recombinant vectors were transfected into DF-1 cells for detecting luciferase activtity, and the core promoter of CBS gene was identified. The results showed that the potential core promoter, classical CpG island and multiple transcription factor Sp1 binding sites were located in 5' regulatory region of CBS gene. All the fragments had promoter activity, and there were significant difference in promoter activity among different fragments. The fragment F4 (-155-+131 bp) with the highest transcription activity was identified as core promoter region. The identification of core promoter and prediction of transcription factor binding sites of CBS gene provide the basis for studying the expression regulatory mechanism of CBS gene in bone.

CLC Number:

S831.2

WANG Han, CHEN Ye, ZHOU Rong-yan, DUNN Ian, CHEN Hui, XI Jian-zhong, ZHANG Zhen-hong. Identification and Analysis of Promoter of Cystathionine Beta Synthase in Layer[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 211-217.

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Identification and Analysis of Promoter of Cystathionine Beta Synthase in Layer

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