一株牛源污蝇杆菌的分离鉴定、耐药性及耐药基因检测

doi:10.11843/j.issn.0366-6964.2017.08.015

Abstract

Abstract:

Wohlfahrtiimonas chitiniclastica is a kind of emerging, rare zoonotic pathogen. In October of 2016, a head of fattening cattle from a beef cattle farm of Jilin Province showed appear purulent lesions in the eyelid, and a variety of antimicrobial treatments are not effective. Eyelid secretion of diseased cattle were collected to isolate and culture bacteria. According to culture, staining, biochemical test and 16S rRNA sequence analysis, the isolated strain was identified as Wohlfahrtiimonas chitiniclastica, and it was pathogenic to mice. The resistance of the isolate was determined by K-B disk diffusion method using 20 antibiotics. The results showed that the isolate was resistant to penicillin, cefradine and erythromycin, and it was sensitive to the other 17 drugs. Then the β-lactam resistance gene (TEM, ADC, OXA-23, OXA-51), macrolide resistance genes (ermA, ermX) and tetracycline resistance genes (tetK, tetW) were detected, respectively, and the results were negative.

CLC Number:

S852.61

LI Xue-song, ZHANG Xi-qing, DONG Wen-long, WANG Yu, ZHANG Hong-wei, TIAN Jia-qi, MA Hong-xia, GAO Yun-hang. Isolation,Identification,Drug Resistance and Detection of Drug Resistance Genes of Bovine Wohlfahrtiimonas chitiniclastica[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1505-1510.

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References

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Isolation,Identification,Drug Resistance and Detection of Drug Resistance Genes of Bovine Wohlfahrtiimonas chitiniclastica

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