15个鸡群体IL-2基因的遗传变异分析

doi:10.11843/j.issn.0366-6964.2015.01.006

Abstract

Abstract:

To deeply study the genetic diversity of chicken Interleukin 2 (IL-2) gene and provide a theoretic basis for the breeding for disease resistance，the genetic variation of IL-2 were studied in 448 individuals from 11 Chinese native，3 introduced and one cultivated chicken breeds by DNA sequencing method in this study.A total of 13 SNPs were identified by using DNA-pooling and sequencing.The results of analysis for a genome region including 5 single nucleotide polymorphisms(SNPs) among all the individuals showed that the frequencies of different alleles differed significantly among 15 populations，suggesting IL-2 had abundant genetic variations and provided promising gene resource for disease resistance.Analysis of several population genetic indexes indicated that the Jingxing Yellow chicken had the lowest level of genetic diversity,which was consistent with its breed formation and little differences of genetic diversity were observed among other breeds.Analysis of haplotypes revealed a total of 23 hapltoyepes.Among them，H1(CAACG)，H2(CAATT)，H4(TGTTG)，H9(TGTTG)，H11(TATCG) and H16(CATCG) were unique or dominant in Chinese native chicken breeds，suggesting these haplotypes may be associated with the disease-resistant ability.

CLC Number:

S831.2

ZHAO Hui-jing，ZHANG Jing-jing,WEN Jie,LIU Ran-ran,ZHENG Mai-qing,LI Qing-he,MA Yue-hui,ZHAO Gui-ping. Genetic Diversity of IL-2 Gene in 15 Chicken Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 41-49.

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References

［1］李海华，胡刚安.优质鸡的抗病育种研究［J］.上海畜牧兽医通讯，2010，(2)：68-69.
LI H H,HU G A.Studies on disease resistance of high quality chicken［J］.Shanghai Journal of Animal Husbandry and Veterinary Medicine,2010,(2):68-69.(in Chinese)
［2］MERLUZZI V，WELTE K，SAVAGE D，et al.Expansion of cyclophosphamide-resistant cytotoxic precursors in vitro and in vivo by purified human interleukin 2［J］.J Immunol，1983，131(2)：806-809.
［3］闫峰宾，康相涛，王彦彬.鸡白细胞介素-2的研究进展及应用前景［J］.广东农业科学，2006，(4)：74-77.
YAN F B，KANG X T，WANG Y B.Chicken interleukin-2 and its foreg round of application［J］.Guangdong Agricultural Sciences，2006，(4)：74-77.（in Chinese）
［4］LIANG Q L，LUO J,ZHOU K，et al.Immune-related gene expression in response to H5N1 avian influenza virus infection in chicken and duck embryonic fibroblasts［J］.Mol Immunol，2011，48(6)：924-930.
［5］WANG Y C，DENG J L，XU S W，et al.Effects of zearalenone on IL-2，IL-6，and IFN-γ mRNA levels in the splenic lymphocytes of chickens［J］.Sci World J，2012，(2012)：1-5.
［6］RAI G D,RAIANATHAN C T M,KUMANNAN K,et al.Changes in the cytokine and toll-like receptor gene expression following infection of indigenous and commercial chickens with infectious bursal disease virus［J］.Ind J Virol，2011，22(2)：146-151.
［7］李宏梅.重组鸡白细胞介素-2 作为免疫增强剂的研究［D］.南京：南京农业大学，2000.
LI H M.Research of recombinant chicken interleukin-2 as an immune enhancer［D］.Nanjing:Nanjing Agricultural University,2000.(in Chinese)
［8］亓丽红，朱剑英，艾武，等.鸡新城疫病毒F基因与鸡白介素-2(IL-2)基因在真核载体中串连表达及免疫原性［J］.农业生物技术学报，2012，20(11)：1327-1332.
QI L H，ZHU J Y，AI W，et al.Tandem-expressing of Newcastle disease virus F and chicken interleukin-2(IL-2) genes in Eukaryotic expression vector and their immunogenicity［J］.Journal of Agricultural Biotechnology，2012，20(11)：1327-1332.(in Chinese)
［9］SONG H，QIU B，YAN R，et al.The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens［J］.Res Vet Sci，2013，94(3)：562-567.
［10］SUNDICK R S,GILL-DIXON C.A cloned chicken lymphokine homologous to both mammalian IL-2 and IL-15［J］. J Immunol，1997，159(2)：720-725.
［11］KAISER P,MARIANI P.Promoter sequence，exon：intron structure，and synteny of genetic location show that a chicken cytokine with T-cell proliferative activity is IL-2 and not IL-15［J］.Immunogenetics，1999，49(1)：26-35.
［12］陈吉刚，周继勇，郑肖娟.中国地方品种丝羽乌骨鸡白细胞介素2基因的克隆及遗传进化分析［J］.中国预防兽医学报，2003，25(1)：21-23.
CHEN J G，ZHOU J Y ,ZHENG X J.Cloning and genetic analysis of interleukin 2 gene in silken feather silky chicken of chinese local breed［J］.Chinese Journal of Preventive Veterinary Medicine，2003，25(1)：21-23.(in Chinese)
［13］王彦彬，卢中华，康相涛，等.固始鸡白细胞介素2基因的克隆,表达及其表达蛋白活性的检测［J］.西北农林科技大学学报(自然科学版)，2006，5(15)：66-71.
WANG Y B，LU Z H ，KANG X T，et al.Cloning and expression of Gushi-chicken interleukin 2 gene and detection of its biological activity［J］.Journal of Northuest A & F University(Natural Science Edition)，2006，5(15)：66-71.(in Chinese)
［14］黄溢泓，谢芝勋，孙建华.广西 10 个地方优质品种鸡白细胞介素2基因的克隆与序列分析［J］.畜牧与兽医，2007，39(1)：7-10.
HUANG Y H,XIE Z X,SUN J H.Molecular cloning and sequencing analysis of interleukin-2 gene from 10 kinds of Guangxi local chickens［J］.Animal Husbandry & Veterinary Medicine,2007,39(1):7-10.(in Chinese)
［15］TOHIDI R,IDRIS I B,PANANDAM J M,et al.The effects of polymorphisms in IL-2，IFN-γ，TGF-β2，IgL，TLR-4，MD-2，and iNOS genes on resistance to Salmonella Enteritidis in indigenous chickens［J］.Avian Pathol，2012，41(6)：605-612.
［16］JOSEPH S,DAVID W.Molecular cloning：a laboratory manual［M］.Cold Spring Harbor Laboratory Press，Cold Spring Harbor，New York，2001：2.
［17］MARUYAMA T.Effective number of alleles in a subdivided population［J］.Theoret Populat Biol，1970，1(3)：273-306.
［18］HORMUZDI S G,PENTTINEN R,JAENISCH R,et al.A gene-targeting approach identifies a function for the first intron in expression of the α1(I) collagen gene［J］.Mol Cell Biol，1998，18(6)：3368-3375.
［19］CHANG K.Critical regulatory domains in intron 2 of a porcine sarcomeric myosin heavy chain gene［J］.J Muscle Res Cell Motil，2000，21(5)：451-461.
［20］ZHANG D P,ZHAO H J，LUO Y Z，et al.Characterization of haplotype diversity defined by discontinuous insertions/Deletions within the Intron 2 of interleukin 2 in different domestic chicken populations［J］.J Biol Sci，2011，11(3)：261-267.
［21］GABRIEL S B,SCHAFFNER S F,NGUYEN H,et al.The structure of haplotype blocks in the human genome［J］.Science，2002，296(5576)：2225-2229.
［22］WU H,BOACKLE S A,HANVIVADHANAKUL P,et al.Association of a common complement receptor 2 haplotype with increased risk of systemic lupus erythematosus［J］.Proc Natl Acad Sci，2007，104(10)：3961-3966.

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Genetic Diversity of IL-2 Gene in 15 Chicken Populations

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