饲粮中添加腐殖酸钠对鼠伤寒沙门菌感染肉鸡肝组织炎症和抗氧化能力的影响

doi:10.11843/j.issn.0366-6964.2024.02.020

摘要/Abstract

摘要： 旨在探究饲粮中添加腐殖酸钠(sodium humate, HNa)对鼠伤寒沙门菌(Salmonella Typhimurium, S. Typhimurium)感染肉鸡肝组织炎症和抗氧化能力的影响。选取健康的1日龄雄性爱拔益加(AA)肉鸡320只,随机分为5组,每组8个重复,每个重复8只鸡:对照组(CON组,基础饲粮)、模型组(ST组,基础饲粮)、低剂量HNa组(L-HNa组,基础饲粮+0.2 g·kg^-1 HNa)、中剂量HNa组(M-HNa组,基础饲粮+0.4 g·kg^-1 HNa)、高剂量HNa组(H-HNa组,基础饲粮+0.6 g·kg^-1 HNa)。在试验第22~24天,CON组肉鸡每天灌胃1 mL PBS,ST、L-HNa、M-HNa和H-HNa组肉鸡每天灌胃1 mL 3×10⁹ CFU·mL^-1 的S. Typhimurium,试验周期24 d。结果显示:1)与ST组相比,饲粮中添加0.6 g·kg^-1的HNa显著提高了肉鸡的平均日增重(P<0.05)。2)与CON组相比,ST组肉鸡肝组织表现为大量炎性细胞浸润,肝脏指数增加,饲粮中添加不同浓度的HNa均缓解了肝组织的病理学损伤(P<0.05)。3)ST组肉鸡血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)的活性显著高于CON组(P<0.05);相较于ST组,L-HNa、M-HNa和H-HNa组肉鸡血清中AKP、ALT和AST的活性显著降低(P<0.05)。4)与CON组相比,ST组肉鸡肝组织过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)和总超氧化物歧化酶(T-SOD)活性显著降低,丙二醛(MDA)含量显著升高(P<0.05);饲粮中添加HNa缓解了S. Typhimurium感染对肉鸡肝组织抗氧化功能的损伤(P<0.05)。5)与ST组相比,饲粮中添加不同浓度的HNa显著降低了肉鸡肝组织白细胞介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)、IL-18、一氧化氮合酶(iNOS)、白细胞分化抗原68(CD68)的mRNA表达,显著增加了IL-10、精氨酸酶-1(ARG1)和CD163的mRNA表达(P<0.05)。6)与CON组相比,S. Typhimurium感染显著提高了肉鸡肝组织核因子κB(NF-κB p65)、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)和半胱天冬蛋白酶-1(Caspase-1)的蛋白表达,降低了核因子κB抑制蛋白α(IκBα)的蛋白表达(P<0.05);饲粮中添加HNa抑制了S. Typhimurium感染引起的肉鸡肝组织NF-κB和NLRP3信号通路的激活。综上所述,饲粮中添加HNa可以通过降低肝组织炎症反应、提高肝抗氧化功能、抑制肝NF-κB和NLRP3信号通路的激活缓解S. Typhimurium感染引起的肉鸡肝损伤。

关键词: 腐殖酸钠, 鼠伤寒沙门菌, 肝组织炎症, 抗氧化, 肉鸡

Abstract: This study was conducted to investigate the effects of dietary supplemented with sodium humate (HNa) on the liver tissue inflammation and antioxidant capacity of Salmonella Typhimurium (S. Typhimurium) infected broilers. A total of 320 healthy 1-day-old Arbor Acres broilers were randomly assigned into 5 treatment groups with 8 replicates of 8 birds each, named CON group (basal diet), ST group (basal diet), L-HNa group (basal diet+0.2 g·kg^-1 HNa), M-HNa (basal diet+0.4 g·kg^-1 HNa), and H-HNa group (basal diet+0.6 g·kg^-1 HNa), respectively. On day 22-24, the broilers in the CON group were gavaged with 1 mL of PBS, while the broilers in the ST, L-HNa, M-HNa and H-HNa groups were gavaged with 1 mL of 3×10⁹ CFU·mL^-1 S. Typhimurium, daily, respectively. The trial lasted for 24 days. The results showed that: 1) Compared with the ST group, dietary supplemented with 0.6 g·kg^-1 HNa significantly increased the average daily gain (ADG) (P<0.05) of broilers. 2) Compared with the CON group, livers of broilers in the ST group exhibited lots of inflammatory cell infiltrations and liver index increased, while dietary supplemented with different concentrations of HNa relieved hepatic histopathological damage (P<0.05). 3) The activities of serum alkaline phosphatase (AKP), alanine aminotransferase (ALT), and aminotransferase (AST) were significantly higher in the ST group broilers compared with the CON group (P<0.05). 4) Compared with the CON group, the broilers in the ST group had lower activities of liver catalase (CAT), superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC) and higher concentration of MDA (P<0.05). Importantly, dietary supplemented with HNa alleviates the damage of S. Typhimurium infection on the antioxidant function of broilers liver. 5) Compared with the ST group, dietary supplemented with different concentrations of HNa significantly decreased the mRNA expression of liver interleukin-1β (IL-1β), IL-18, tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), and cluster of differentiation 68 (CD68) (P<0.05), while significantly increased the mRNA expression of IL-10, arginase-1 (ARG1), and cluster of differentiation 163 (CD163)(P<0.05). 6) Compared with the CON group, S. Typhimurium infection significantly elevated the protein expression of nuclear factor kappa beta p65 (NF-κB p65), NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and Caspase-1 (P<0.05), significantly decreased the protein expression of NF-kappa-B inhibitor alpha (IκBα)(P<0.05). However, dietary administration of HNa inhibited the activation of NF-κB and NLRP3 signaling pathway. In summary, dietary supplementation of HNa alleviated liver damage of broilers caused by S. Typhimurium infection by decreasing liver inflammation response, increasing liver antioxidant function and suppressing activation of NF-κB and NLRP3 signaling pathway in the liver.

Key words: sodium humate, Salmonella Typhimurium, liver inflammation, antioxidant, broiler

中图分类号:

S816.7

王栋, 柳可欣, 何炎峻, 邓守翔, 刘云, 马卫明. 饲粮中添加腐殖酸钠对鼠伤寒沙门菌感染肉鸡肝组织炎症和抗氧化能力的影响[J]. 畜牧兽医学报, 2024, 55(2): 629-639.

WANG Dong, LIU Kexin, HE Yanjun, DENG Shouxiang, LIU Yun, MA Weiming. Effects of Dietary Sodium Humate Supplementation on Liver Tissue Inflammation and Antioxidant Capacity of Salmonella Typhimurium-Infected Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 629-639.

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