盐酸不溶灰分测定方法影响肉鸡饲粮代谢能准确性的比较研究

doi:10.11843/j.issn.0366-6964.2024.02.019

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (2): 619-628.doi: 10.11843/j.issn.0366-6964.2024.02.019

盐酸不溶灰分测定方法影响肉鸡饲粮代谢能准确性的比较研究

宋明强¹, 解竞静¹, 欧娟², 王钰明¹, 侯嘉², 谭高明¹, 田凯¹, 朱云¹, 萨仁娜¹, 赵峰^1*

1. 中国农业科学院北京畜牧兽医研究所畜禽营养与饲养重点实验室, 北京 100193;
2. 杭州康德权饲料有限公司包膜饲料添加剂省级重点农业企业研究院, 杭州 311107

收稿日期:2023-08-05 出版日期:2024-02-23 发布日期:2024-02-27
通讯作者: 赵峰,主要从事单胃动物饲料养分生物学效价评定研究,E-mail:zhaofeng@caas.cn
作者简介:宋明强(1998-),男,山东沂水人,硕士生,主要从事家禽饲料养分生物学效价评定研究,E-mail:Song15263936064@163.com
基金资助:
国家自然科学基金(32172762);现代农业产业技术体系北京市家禽创新团队(BAIC06-2022)

Comparative Study on the Accuracy of Dietary Metabolizable Energy Influenced by Methods of Acid Insoluble Ash in Broilers

SONG Mingqiang¹, XIE Jingjing¹, OU Juan², WANG Yuming¹, HOU Jia², TAN Gaoming¹, TIAN Kai¹, ZHU Yun¹, SA Renna¹, ZHAO Feng^1*

1. State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Provincial Key Agricultural Enterprise Research Institute of Encapsulated Feed Additive, King Techina Technology Co., Ltd, Hangzhou 311107, China

Received:2023-08-05 Online:2024-02-23 Published:2024-02-27

摘要/Abstract

摘要： 旨在改进美国油脂化学家协会(The American Oil Chemists' Society,简称AOCS)测定盐酸不溶灰分(AIA)标准方法的上样量和过滤介质,以简化测定步骤、提高测定精度,并通过AOCS改进法与AOCS标准方法测定AIA,比较两种方法的重复性、可加性及准确性,为准确评价肉鸡对饲粮的代谢能(AME)提供可靠的技术。本研究分为两个试验。试验一采用单因素完全随机设计,通过两种方法(AOCS改进法、AOCS标准方法)对6个样品(3个饲粮、3个排泄物)分3个批次测定AIA含量,比较两种测定方法的可重复性;然后,通过两种方法测定6个混合饲粮和6个混合排泄物样品AIA的实际含量,同时,根据混合饲粮和混合排泄物的组成及AIA含量得到其AIA的计算值,采用配对设计比较实测值与计算值的差异以检验两种测定方法的可加性。试验二采用配对试验设计,比较两种方法测定AIA计算13个饲粮AME与全收粪法测定AME的差值,验证两种方法评价饲粮AME的准确性。结果表明,AOCS改进法测定3个饲粮和3个排泄物AIA的总变异系数(TCV)分别在2.13%~5.83%和1.71%~2.83%,而AOCS标准方法测定上述样品AIA的TCV分别在4.69%~15.37%和1.50%~5.79%;AOCS改进法测定6个混合饲粮和混合排泄物AIA实测值和计算值的差值分别在0.004%~0.017%和0.002%~0.029%,而AOCS标准方法测定上述样品实测值和计算值的差值分别在0.037%~0.071%和0.015%~0.072%。AOCS改进法测定AIA评价饲粮AME与全收粪法AME测值相差在0.021~0.251 MJ·kg^-1 DM,而AOCS标准方法测定AIA评价饲粮AME与全收粪法AME测值相差0.025~1.799 MJ·kg^-1 DM。综上所述,从测定AIA的重复性、可加性及对AME测定值的准确性上,AOCS改进法均优于AOCS标准方法。

关键词: 盐酸不溶灰分, 测定方法, 代谢能, 肉鸡

Abstract: The objective of this study was to optimize the loading sample size and filter medium of The American Oil Chemists' Society (AOCS) standard method for the determination of acid insoluble ash (AIA) to simplify the assay procedure and improve the accuracy. The repeatability, additivity and accuracy of AIA determined with AOCS modified method and the AOCS standard method were compared to obtain a reliable technique for accurately evaluating the dietary apparent metabolizable energy (AME) for broilers. Two experiments were conducted in the current study. In experiment 1, three batches determination of AIA content for each of 6 samples (3 diets, 3 excreta) using two methods (AOCS modified method, AOCS standard method) in a completely randomized design was to compare the reproducibility of the 2 methods. Then, the actual AIA content of 6 mixed diets and 6 mixed excreta samples were determined by the 2 methods. Meanwhile, the AIA value was calculated according to the composition of the mixed diet and mixed excreta and their AIA content. The difference between the determined and the calculated values was compared to test the additivity of the 2 methods by a paired experimental design. In experiment 2, a paired experimental design was used to compare the difference between the calculated AME of 13 diets using two AIA determination methods and the AME measured by the total collection method to test the accuracy of the 2 methods to evaluate the dietary AME. The results showed that the total coefficient of variation (TCV) of AIA determined by the AOCS modified method in each of 3 diets and 3 excreta samples ranged from 2.13% to 5.83% and 1.71% to 2.83%, respectively, while the TCV of AIA determined by the AOCS standard method ranged from 4.69% to 15.37% and 1.50% to 5.79%, respectively. The difference between the determined and calculated AIA values of 6 mixed diets and 6 mixed excreta by the AOCS modified method ranged from 0.004% to 0.017% and 0.002% to 0.029%, respectively. The difference between determined and calculated values of those samples by AOCS standard method ranged from 0.037% to 0.071% and 0.015% to 0.072%, respectively. The differences of 0.021 to 0.251 MJ·kg^-1 DM were observed between the AME evaluated by AIA determined with the AOCS modified method and AME determined by total collection. Whereas the differences of 0.025 to 1.799 MJ·kg^-1 DM were observed between AME evaluated by AIA determined with the AOCS standard method and AME determined by total collection. In conclusion, the AOCS modified method is superior to the AOCS standard method in the reproducibility and additivity of AIA determination and in the accuracy of evaluating dietary AME.

Key words: acid insoluble ash, determined method, metabolizable energy, broiler

中图分类号:

S816.17

宋明强, 解竞静, 欧娟, 王钰明, 侯嘉, 谭高明, 田凯, 朱云, 萨仁娜, 赵峰. 盐酸不溶灰分测定方法影响肉鸡饲粮代谢能准确性的比较研究[J]. 畜牧兽医学报, 2024, 55(2): 619-628.

SONG Mingqiang, XIE Jingjing, OU Juan, WANG Yuming, HOU Jia, TAN Gaoming, TIAN Kai, ZHU Yun, SA Renna, ZHAO Feng. Comparative Study on the Accuracy of Dietary Metabolizable Energy Influenced by Methods of Acid Insoluble Ash in Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 619-628.

参考文献

[1] SCOTT T A,HALL J W.Using acid insoluble ash marker ratios (diet:digesta) to predict digestibility of wheat and barley metabolizable energy and nitrogen retention in broiler chicks[J].Poult Sci,1998,77(5):674-679.
[2] DAVID L S,ABDOLLAHI M R,RAVINDRAN G,et al.Studies on the measurement of ileal calcium digestibility of calcium sources in broiler chickens[J].Poult Sci,2019,98(11):5582-5589.
[3] PRAWIRODIGDO S,GANNON N J,LEURY B J,et al.Acid-insoluble ash is a better indigestible marker than chromic oxide to measure apparent total tract digestibility in pigs[J].Anim Nutr,2021,7(1):64-71.
[4] SONG D,LI A K,WANG Y W,et al.Effects of synbiotic on growth,digestibility,immune and antioxidant performance in broilers[J].Animal,2022,16(4):100497.
[5] DIAS K M M,OLIVEIRA C H,CALDERANO A A,et al.Research note:nitrogen-corrected apparent metabolizable energy and standardized ileal amino acid digestibility determination of high-protein DDG and corn bran with solubles for broilers[J].Poult Sci,2023,102(7):102757.
[6] GREENHALGH S,MACELLINE S P,CHRYSTAL P V,et al.An evaluation of elevated branched-chain amino acid inclusions on the performance of broiler chickens offered reduced-crude protein,wheat-based diets from 7 to 28 days post-hatch[J].Anim Feed Sci Technol,2022,286:115255.
[7] 中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 23742-2009 饲料中盐酸不溶灰分的测定[S].北京:中国标准出版社,2009.
General Administration of Quality Supervision,Inspection and Quarantine of the People's Republic of China,Standardization Administration of the People's Republic of China.GB/T 23742-2009 Animal feeding stuffs-Determination of ash insoluble in hydrochloric acid[S].Beijing:Standards Press of China,2009.(in Chinese)
[8] AOCS.Acid-insoluble ash (Ba 5b-68)[S].American Oil Chemists Society,2009.
[9] SALES J,JANSSENS G P J.Acid-insoluble ash as a marker in digestibility studies:a review[J].J Anim Feed Sci,2003,12(3):383-401.
[10] 王钰明,赵峰,陈寿飞,等.猪生长阶段与饲粮类型对酸不溶灰分法测定养分消化率的影响[J].动物营养学报,2015,27(3):811-819.
WANG Y M,ZHAO F,CHEN S F,et al.Effects of growing phase and diet type on determination of nutrient digestibility by acid insoluble ash technique for pigs[J].Chinese Journal of Animal Nutrition,2015,27(3):811-819.(in Chinese)
[11] SONG M Q,WANG Y M,LIU Y Y,et al.The age-related metabolizable energy of cereal grains,oilseed meals,corn gluten meals,and feather meals for broilers[J].J Anim Sci,2023,101:skad051.
[12] LATIFI M,MORAVEJ H,GHAZIANI F,et al.Determination of prediction equations for apparent metabolizable energy corrected for nitrogen of corn gluten meal and canola meal in broilers[J].Poult Sci,2023,102(5):102587.
[13] 蒋红卫,夏结来.基于样本变异系数的组间与组内变异统计量[C]//2006年中国卫生统计学术交流大会论文集.武汉:中国卫生信息学会,2006:1-7.
JIANG H W,XIA J L.Inter-group and intra-group variation statistics based on coefficient of variation of samples[C]//Proceedings of the 2006 China Health Statistics Academic Exchange Conference.Wuhan:Chinese Health Information Association,2006:1-7.(in Chinese)
[14] LIU K S.New and improved methods for measuring acid insoluble ash[J].Anim Feed Sci Technol,2022,288:115282.
[15] 杜中原,苏艳芳,陈凯旋,等.2种氧弹热量计测定样品总能的差异及其对能量消化率的影响[J].动物营养学报,2021,33(2):1128-1136.
DU Z Y,SU Y F,CHEN K X,et al.Difference in two types of oxygen bomb calorimeter to determine gross energy and its effects on energy digestibility of samples[J].Chinese Journal of Animal Nutrition,2021,33(2):1128-1136.(in Chinese)
[16] 廖睿,赵峰,张虎,等.仿生消化法测定猪饲料原料还原糖释放量的重复性和可加性研究[J].动物营养学报,2017,29(1):168-176.
LIAO R,ZHAO F,ZHANG H,et al.A study on the repeatability and additivity of the released amount of reducing sugar of feed ingredients determined with simulated digestion method for pigs[J].Chinese Journal of Animal Nutrition,2017,29(1):168-176.(in Chinese)
[LL] [17] YU Y,ZHAO F,CHEN J,et al.Sensitivity of in vitro digestible energy determined with computer-controlled simulated digestion system and its accuracy to predict dietary metabolizable energy for roosters[J].Poult Sci,2021,100(1):206-214.
[18] SIBBALD I R.A bioassay for true metabolizable energy in feedingstuffs[J].Poult Sci,1976,55(1):303-308.

盐酸不溶灰分测定方法影响肉鸡饲粮代谢能准确性的比较研究

Comparative Study on the Accuracy of Dietary Metabolizable Energy Influenced by Methods of Acid Insoluble Ash in Broilers

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