不同猪支原体肺炎活疫苗菌株P46和P97基因序列差异性分析

doi:10.11843/j.issn.0366-6964.2017.12.016

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (12): 2365-2373.doi: 10.11843/j.issn.0366-6964.2017.12.016

不同猪支原体肺炎活疫苗菌株P46和P97基因序列差异性分析

武昱孜¹, 邢宪平^1,2, 韦艳娜¹, 熊祺琰¹, 冯志新¹, 邵国青^1,3*

1. 江苏省农业科学院兽医研究所农业部兽用生物制品工程技术重点实验室/国家兽用生物制品工程技术研究中心, 南京 210014;
2. 南京农业大学, 南京 210095;
3. 肉类生产与加工质量安全控制协同创新中心, 南京 210095

收稿日期:2017-06-13 出版日期:2017-12-23 发布日期:2017-12-23
通讯作者: 邵国青,E-mail:gqshaonj@163.com
作者简介:武昱孜(1984-),女,山西太谷人,硕士,主要从事生物化学与分子生物学研究,E-mail:zzzizi24@163.com
基金资助:
江苏省农业科技自主创新资金项目（CX （14）2040）；国家自然科学基金面上项目（31370208）

Comparison of Nucleotide and Deduced Amino Acid Sequences of P46 and P97 for the Different Live Vaccine Strains of Mycoplasmal Pneumonia of Swine

WU Yu-zi¹, XING Xian-ping^1,2, WEI Yan-na¹, XIONG Qi-yan¹, FENG Zhi-xin¹, SHAO Guo-qing^1,3*

1. Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Nanjing Agricultural University, Nanjing 210095, China;
3. Jiangsu Collaborative Innovation Center for Meat Production, Processing and Quality Control, Nanjing 210095, China

Received:2017-06-13 Online:2017-12-23 Published:2017-12-23

摘要/Abstract

摘要：

对我国用于生产的3种猪支原体肺炎活疫苗菌株（168株、RM48株、Z株）进行P46和P97R1R2基因序列分析，考察其保守性和差异。提取来源于四家猪支原体肺炎活疫苗生产企业的疫苗株基因组DNA，以其为模板进行PCR扩增、测序；利用DNAStar序列分析软件对测序结果和国际标准株J株、232株、7448株、7422株一起进行比对，分析3种疫苗菌株和4个标准株之间核苷酸及氨基酸差异。结果发现，疫苗株Z株、RM48株、168株的P46基因序列相似性为100%，与J株、232株相似性为99.1%，与7422株、7448株相似性为98.9%。疫苗株RM48株和Z株的P97R1R2基因序列相似性为100%；而与疫苗株168株相似性为97.5%；三个疫苗株与J株、232株、7448株、7422株的P97R1R2基因相似性仅为90.1%~93.6%，差异主要发生在R1区重复序列的次数上。本研究为临床猪支原体肺炎活疫苗菌株的检测及不同菌株之间的差异鉴定研究奠定了基础。

Abstract:

This experiment was conducted to study the conservation and variation of P46 gene and P97R1R2 gene of three Mycoplasmal pneumonia of swine vaccine strains, e.g.168, RM48 and Z, from domestic production. The DNA of the four vaccine manufacturer products were extracted. The target gene fragments amplified by PCR were sequenced. And the nucleotides sequence and the deduced amino acids sequences were analyzed by DNAStar software, which compared with four international standard strains.The attenuated vaccine strain 168 showed 100% homology of P46 nucleotides sequence with vaccine strain Z and RM48, while 99.1% of that with strain J and 232, 98.9% of that with strain 7422 and 7448. As for P97R1R2 gene, strain Z and RM48 showed completely same identity, and 97.5% of identity with strain 168. The homology among vaccine strains, strain J, 232, 7448 and strain 7422 was lower, only from 90.1% to 93.6%. The major differences of the vaccine strains occurred in the repeat numbers of P97R1 amino acid sequence, which is benefit for differentiation of the attenuated vaccine strains.

中图分类号:

S852.62

武昱孜, 邢宪平, 韦艳娜, 熊祺琰, 冯志新, 邵国青. 不同猪支原体肺炎活疫苗菌株P46和P97基因序列差异性分析[J]. 畜牧兽医学报, 2017, 48(12): 2365-2373.

WU Yu-zi, XING Xian-ping, WEI Yan-na, XIONG Qi-yan, FENG Zhi-xin, SHAO Guo-qing. Comparison of Nucleotide and Deduced Amino Acid Sequences of P46 and P97 for the Different Live Vaccine Strains of Mycoplasmal Pneumonia of Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2365-2373.

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不同猪支原体肺炎活疫苗菌株P46和P97基因序列差异性分析

Comparison of Nucleotide and Deduced Amino Acid Sequences of P46 and P97 for the Different Live Vaccine Strains of Mycoplasmal Pneumonia of Swine

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