尾分析法在不同规模群体中开展全基因组关联研究

doi:10.11843/j.issn.0366-6964.2017.07.002

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (7): 1181-1190.doi: 10.11843/j.issn.0366-6964.2017.07.002

尾分析法在不同规模群体中开展全基因组关联研究

武群清^1,2, 张龙超², 黄生强^1,3*, 王立贤^2*

1. 湖南农业大学动物科学技术学院, 长沙 410128;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
3. 湖南省畜禽安全生产协同创新中心, 长沙 410128

收稿日期:2016-12-12 出版日期:2017-07-23 发布日期:2017-07-23
通讯作者: 黄生强,副教授,E-mail:hsq07@126.com;王立贤,研究员,E-mail:iaswlx@263.net
作者简介:武群清(1991-),男,湖南怀化人,硕士生,主要从事动物遗传育种与繁殖方面的研究,E-mail:292566984@qq.com
基金资助:
国家科技支撑计划项目（2015BAD03B02-2）；中国农业科学院科技创新工程（ASTIP-IAS02）；国家生猪产业技术体系（CARS-36）

A Genome-wide Association Study of Different Size Populations Based on Tail Analysis

WU Qun-qing^1,2, ZHANG Long-chao², HUANG Sheng-qiang^1,3*, WANG Li-xian^2*

1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
3. Hunan Co-innovation Center of Animal Production Safety, Changsha 410128, China

Received:2016-12-12 Online:2017-07-23 Published:2017-07-23

摘要/Abstract

摘要：

旨在将尾分析法与全基因组关联分析相结合，初步确定两尾选择的标准。首先，对样本量为2 000、1 500、1 000、500的群体进行全基因组关联分析（GWAS），探索群体规模大小对GWAS结果的影响，然后对各样本量两尾的20%、15%、12%、10%、8%、5%进行GWAS，探索两尾比例的大小对GWAS结果的影响。研究结果表明，检测出的显著关联SNP数目随群体规模及两尾比例的减小而减少。初步确定两尾选择标准为：遗传力为0.38左右的性状，样本量为2 000、1 500、1 000、500，两尾选择比例分别为8%、10%、10%、20%；遗传力为0.50左右的性状，样本量为2 000、1 500、1 000、500，两尾选择比例分别为5%、5%、10%、15%。以此标准为基础进行GWAS，既能有效检测出最显著SNP位点或区间，又能最大程度地降低研究成本，提高研究效率。

Abstract:

Tail analysis and genome-wide association studies(GWAS) were combined in this study to determine preliminary two tails selection criterions. A total of 2 000, 1 500, 1 000 and 500 samples and 20%, 15%, 12%, 10%, 8%, 5% of two tails were used to run GWAS to estimate the effects of the population size on GWAS and the effect of the proportion of two tails on GWAS, respectively. The results showed that the number of significant SNPs decreased with the reducing population size and the proportion of two tails. The preliminary selection criterions were:traits with heritability of about 0.38, the proportion of two tails of 8%, 10%, 10% and 20% from 2 000, 1 500, 1 000 and 500 samples, which were appropriate for two tails population for running GWAS, respectively. Further, the traits with heritability of about 0.50, the proportion of two tails of 5%, 5%, 10% and 15% from 2 000, 1 500, 1 000 and 500 samples were appropriate for two tails population for running GWAS, respectively. The present preliminary two tails selection criterions from populations with various sizes to run GWAS could detect significant SNPs effectively and also provide a strategy of reducing the cost of GWAS.

中图分类号:

S828
S813.1

武群清, 张龙超, 黄生强, 王立贤. 尾分析法在不同规模群体中开展全基因组关联研究[J]. 畜牧兽医学报, 2017, 48(7): 1181-1190.

WU Qun-qing, ZHANG Long-chao, HUANG Sheng-qiang, WANG Li-xian. A Genome-wide Association Study of Different Size Populations Based on Tail Analysis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1181-1190.

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尾分析法在不同规模群体中开展全基因组关联研究

A Genome-wide Association Study of Different Size Populations Based on Tail Analysis

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