藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析

doi:10.11843/j.issn.0366-6964.2017.07.003

摘要/Abstract

摘要：

旨在获得藏鸡FTO基因序列，并分析其生物学特性，阐明其组织及时序表达规律，同时揭示该基因在不同发育阶段肌肉组织中的表达水平与肌内脂肪含量的关系。选取1~210日龄健康藏鸡为试验材料，采集心、肝、脾、肺、肾、胸肌、腿肌和皮下脂肪组织样品，提取组织总RNA，利用RT-PCR方法克隆FTO基因序列，同时利用实时荧光定量PCR检测其在各组织及不同发育阶段的mRNA表达丰度，并将其表达量与肌内脂肪含量进行相关分析。结果表明，获得藏鸡FTO的基因序列长1 585 bp（GenBank登陆号：KY366175），其中CDS为1 524 bp，5'UTR 35 bp和3'UTR 26 bp，编码507个氨基酸，具有一个N端结构域和一个C端结构域。藏鸡与原鸡FTO氨基酸相比发生了6个氨基酸突变。FTO基因在藏鸡的各个组织中广泛表达，在脾组织中的表达水平极显著高于其他组织（P<0.01），在脂肪组织和肺组织也存在较高水平的表达。时序表达谱显示，FTO基因在藏鸡胸肌和腿肌具有不同的表达模式，在胸肌中的表达水平随生长日龄的增加呈逐渐下降的趋势，而在腿肌中的表达水平随生长日龄的增加呈先上升后下降的趋势。藏鸡在不同发育阶段，不同肌肉组织中FTO基因表达水平与IMF含量具有不同程度的相关性，其中FTO基因表达水平与藏鸡胸肌IMF含量呈负相关，并且在母鸡中的相关性达到显著水平（P<0.05）。而公鸡FTO基因表达水平与腿肌IMF含量呈正相关（在119~210日龄阶段r=0.601，P<0.05），母鸡则相反。研究结果提示，FTO基因可能在藏鸡生长发育过程中对肌内脂肪沉积起重要调控作用，本试验结果将为藏鸡FTO基因的结构和功能的进一步研究奠定基础。

Abstract:

The aim of this study was to obtain the sequence of FTO gene of Tibetan chicken, analyze its sequence by bioinformatics, clarify its temporal-spatial expression characteristics, and reveal the relationship between the expression level of FTO gene and intramuscular fat content(IMF) at different developmental stages. Healthy Tibetan chickens in various ages(1-210 days of age) were selected, the tissue samples of heart, liver, spleen, lung, kidney, breast muscle, leg muscle and subcutaneous fat were collected for the total RNA extraction. The Reverse Transcription PCR(RT-PCR) was used to clone Tibetan chicken FTO gene sequence, the real-time quantitative PCR(qPCR) was used to detect the expression level of FTO gene in different tissues and developmental stages, and the correlation between the FTO mRNA expression and intramuscular fat content was analyzed. The results showed that the sequence of Tibetan chicken FTO gene was 1 585 bp (GenBank accession number:KY366175), containing 1 524 bp CDS, 35 bp 5' UTR and 26 bp 3' UTR, encoding 507 amino acids with a N-terminal domain and a C-terminal domain. There were 6 amino acid mutations between Tibetan chicken and Gallus gallus FTO amino acid sequences. FTO gene of Tibetan chicken was widely expressed in different tissues. The expression level of FTO gene was the highest in spleen, which was significantly higher than other tissues (P <0.01). FTO gene also kept a high expression level in adipose and lung tissues. The expression pattern of Tibetan chicken FTO gene in breast muscle and leg muscle were different, the expression level in breast muscle decreased gradually with the growth of Tibetan chicken, while in the leg muscle, the expression level increased first and then descended with the increasing age. The expression levels of Tibetan chickenFTO gene in different muscle tissues and different developmental stages presented diverse relationships with IMF content. The expression level of FTO in breast muscle of Tibetan chicken was negatively correlated with IMF content, and this correlation in hens was significantly stronger (P <0.05). In leg muscle, the expression level of FTO gene in cocks was positively correlated with IMF content(in period of 119-210 days, r=0.601, P <0.05), while in hens this relationship was opposite. The results suggest that FTO gene may play an important role in intramuscular fat deposition during the growth and development stage of Tibetan chicken. These results will facilitate the research on the structure and function of FTO gene in Tibetan chickens.

中图分类号:

S831.2

林森, 林亚秋, 朱江江, 徐亚欧, 王永. 藏鸡FTO基因表达的时空特性及与肌内脂肪含量的相关分析[J]. 畜牧兽医学报, 2017, 48(7): 1191-1201.

LIN Sen, LIN Ya-qiu, ZHU Jiang-jiang, XU Ya-ou, WANG Yong. Temporal-Spatial Characteristics of FTO Gene Expression in Tibetan Chicken and Its Correlation with Intramuscular Fat Content[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(7): 1191-1201.

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