CRTC3基因多态性及基因型组合与秦川牛生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2016.11.005

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (11): 2184-2190.doi: 10.11843/j.issn.0366-6964.2016.11.005

CRTC3基因多态性及基因型组合与秦川牛生长性状的关联分析

徐怀超¹,昝林森^1,2*,王洪宝^1,2,宁越¹

(1.西北农林科技大学动物科技学院, 杨凌 712100； 2. 国家肉牛改良中心, 杨凌 712100)

收稿日期:2016-02-29 出版日期:2016-11-23 发布日期:2016-11-23
通讯作者: 昝林森，教授，主要从事秦川肉牛新品系选育、肉牛遗传改良与种质创新等方面的研究，E-mail: zanlinsen@163.com
作者简介:徐怀超(1989-)，男，山东聊城人，硕士生，主要从事动物生物技术研究， E-mail: 727751348@qq.com
基金资助:
国家863计划（2013AA102505）；国家科技支撑计划（2015BAD03B04）；国家自然科学基金（31272411）；国家肉牛牦牛产业技术体系（CARS-38）；陕西省科技统筹创新工程计划（2014KTZB02-02-01）

Association of CRTC3 Gene Polymorphisms and Genotype Combination with Growth Traits of Qinchuan Cattle

XU Huai-chao¹,ZAN Lin-sen^1,2* ,WANG Hong-bao^1,2,NING Yue¹

(1. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China;2. National Beef Cattle Improvement Center, Yangling 712100, China)

Received:2016-02-29 Online:2016-11-23 Published:2016-11-23

摘要/Abstract

摘要：

旨在探讨CRTC3基因作为秦川牛生长性状候选基因的可能性，寻找与秦川牛生长相关的分子标记。本研究采用PCR-RFLP方法检测395头健康秦川牛CRTC3基因的多态性，分析其多态位点不同基因型及组合基因型与秦川牛生长性状的关联性。结果发现，CRTC3基因扩增序列区间存在2个SNPs位点（位于外显子区域的G66478C和位于内含子区域的C91297T）。关联性分析表明，在本试验所选取的395头秦川牛群体中， G66478C位点GC基因型个体在体斜长方面极显著高于CC型个体均值（P＜0.01），且在胸深方面显著高于CC型个体均值（P＜0.05）。在C91297T位点，CT基因型个体均值在体斜长、腰高、尻长和胸深方面显著高于TT基因型个体均值（P＜0.01）。CRTC3基因的优势基因型组合CC-TT的个体在腰高、尻长上极显著高于CC-CT基因型组合的个体均值（P<0.01），且基因型组合CC-TT的个体在胸深、胸围显著高于CC-CT组合的个体均值（P<0.05）。综上，可以尝试将CRTC3基因作为影响秦川牛生长性状的候选基因用于标记辅助选择，为秦川牛选育工作提供科学依据。

Abstract:

The aim of this study was to investigate the possibility of CRTC3 gene as candidate gene for growth performance in Qinchuan cattle, and search the molecular markers related to Qinchuan cattle growth traits. PCR-RFLP method was applied to detect the polymorphisms of CRTC3 in 395 healthy Qinchuan cattle, and the association of its polymorphisms and genotype combination with growth traits of Qinchuan cattle was also analyzed. The result showed that 2 SNPs were identified, including G66478C in exon and C91297T in intron. At the G66478C locus, the cattle with genotype GC had greater body length and chest depth values than those with genotype CC(P<0.01 and P<0.05, respectively). At the C91297T locus, animals with genotype CT had greater body length, hip height, rump length and chest depth values than those with genotype TT(P < 0.01) . Animals with CC-TT had significantly greater hip height and rump length values than those with CC-CT(P < 0.01), and chest depth and chest circumference values for CC-TT were greater than CC-CT(P<0.05). Therefore, variations in the CRTC3 gene and the genotype combination CC-CT may be useful as molecular markers for growth traits in Qinchuan cattle breeding.

中图分类号:

S823
S813.3

徐怀超,昝林森,王洪宝,宁越. CRTC3基因多态性及基因型组合与秦川牛生长性状的关联分析[J]. 畜牧兽医学报, 2016, 47(11): 2184-2190.

XU Huai-chao,ZAN Lin-sen,WANG Hong-bao,NING Yue. Association of CRTC3 Gene Polymorphisms and Genotype Combination with Growth Traits of Qinchuan Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2184-2190.

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CRTC3基因多态性及基因型组合与秦川牛生长性状的关联分析

Association of CRTC3 Gene Polymorphisms and Genotype Combination with Growth Traits of Qinchuan Cattle

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