务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析

doi:10.11843/j.issn.0366-6964.2013.08.005

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (8): 1205-1212.doi: 10.11843/j.issn.0366-6964.2013.08.005

务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析

杨永强¹，焦仁刚²，龚俞²，谢海强¹，惠嫣婷¹，张依裕¹，肖超能¹，刘若余^1*

（1. 贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室，贵阳 550025； 2. 贵州省畜牧技术推广站，贵阳 550001）

收稿日期:2013-01-14 出版日期:2013-08-23 发布日期:2013-08-23
通讯作者: 刘若余，E-mail：liury04@163.com
作者简介:杨永强(1989-)，男，四川威远人，硕士生，主要从事分子遗传与动物育种研究，E-mail：yangyongqiang2000@126.com
基金资助:
贵州省重大科技专项计划项目（黔科合重大专项字［2011］6009号）

Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle

YANG Yong-qiang¹, JIAO Ren-gang², GONG Yu², XIE Hai-qiang¹, HUI Yan-ting¹, ZHANG Yi-yu¹, XIAO Chao-neng¹, LIU Ruo-yu^1*

(1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education/ Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Animal Science and Technology Station of Guizhou, Guiyang 550001, China)

Received:2013-01-14 Online:2013-08-23 Published:2013-08-23

摘要/Abstract

摘要：

为筛选STAT3基因启动子区SNPs及研究其对启动子功能元件和体尺性状的影响。选择品种差异较大的贵州荷斯坦奶牛和务川黑牛构建不同DNA池，直接测序筛选SNP位点，PCR-RFLP进行验证和基因分型，并利用114头务川黑牛个体分析SNPs位点与8个体尺性状的相关性。结果表明：STAT3基因5′调控区存在7个新SNPs位点，分别为：G-449A、C-341A、A-322G、C-246A、G-240A、C-225T、A-103T，提交SNP数据库得到登录号。生物信息学软件预测得到STAT3基因核心启动子区和CpG岛，SNP位点导致附近大量转录因子结合位点消失和新位点产生。多态性位点对转录因子结合位点有显著影响，但并不改变CpG岛大小。关联性分析结果表明，A-322G对务川黑牛体重有极显著影响（P<0.01），A等位基因为有利等位基因。与AA或GG纯合基因型相比，AG基因型个体有更出色的体重性状。A-322G可能作为影响肉牛生长性状的重要功能性SNP，STAT3基因可能作为肉牛选种选育的候选基因，研究结果为进一步确定STAT3基因功能奠定试验基础。

Abstract:

The study aimed to screen the SNPs in the promoter region of STAT3 gene in cattle and analyze the effect of SNPs on function elements of promoter and growth traits. Two cattle breeds (Wuchuan Black cattle and Guizhou Holstein cow) that possessed significant difference in breeds property were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently. PCR-RFLP was used to be genotyping and a total of 114 Wuchuan Black cattle were used to study the associations of SNPs with 8 growth traits. Results showed that 7 new single nucleotide polymorphism (SNP) sites including G-449A, C-341A, A-322G, C-246A, G-240A, C-225T and A-103T, respectively were found in the 5' flanking region of STAT3 gene and then submitted the information of SNPs to NCBI to obtain accession numbers. Furthermore, bioinformatics tools were used to predict the core region of the promoter and CpG island. SNPs found in this study led to the disappearance of a large amount of transcription factors bingding sites and the appearance of new sites, it also showed that SNP mutation didn’t affect the range of CpG island dramaticlly by using various softwares. Association analysis demonstrated that A-322G had highly significant effects on body weight (P<0.01), A allele was the favorable alleles. Moreover, animals with the genotype AG had the highest mean body weight than those with GG and AA genotypes, which suggested that A-322G was an important SNP marker affecting the cattle growth performance and STAT3 gene could be applied in marker-assisted selection for improving growth performance in cattle. This work could lay the experimental foundation for the identification of function of STAT3 gene in cattle.

中图分类号:

S823
S813.3

杨永强，焦仁刚，龚俞，谢海强，惠嫣婷，张依裕，肖超能，刘若余. 务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析[J]. 畜牧兽医学报, 2013, 44(8): 1205-1212.

YANG Yong-qiang, JIAO Ren-gang, GONG Yu, XIE Hai-qiang, HUI Yan-ting, ZHANG Yi-yu, XIAO Chao-neng, LIU Ruo-yu. Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1205-1212.

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参考文献

［1］CALO V, MIGLIAVACCA M, BAZAN V, et al. STAT proteins: from normal control of cellular events to tumorigenesis ［J］. J Cell Physiol, 2003, 197(2):157-168.

［2］LANNING N J, CARTER-SU C. Recent advances in growth hormone signaling ［J］. Rev Endocr Metab Disorders, 2006, 7(4):225-235.

［3］HERRINGTON J, SMIT L S, SCHWARTZ J, et al. The role of STAT proteins in growth hormone signaling ［J］. Oncogene, 2000, 19(21):2585-2597.

［4］BUETTNER R, MORA L B, JOVE R. Activated STAT signaling in human tumors provides novel molecular targets for therapeutic intervention ［J］. Clin Cancer Res, 2002, 8(4):945-954.

［5］HIRANO T, ISHIHARA K, HIBI M. Roles of STAT3 in mediating the cell growth, differentiation and survival signals relayed through the IL-6 family of cytokine receptors ［J］. Oncogene, 2000, 19(21):2548-2556.

［6］MOLENAAR A, WHEELER T, MCCRACKEN J, et al. The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A-and STAT5B-encoding genes in cattle ［J］. Anim Genet, 2000, 31(5):339.

［7］MEYER L, BROCKMANN G, SCHWERIN M, et al. A highly polymorphic GT-repeat in the bovine STAT5B encoding gene on BTA19 ［J］. Anim Genet, 1999, 30(1):72-73.

［8］BILD A H, TURKSON J, JOVE R. Cytoplasmic transport of Stat3 by receptor-mediated endocytosis ［J］. The EMBO J, 2002, 21(13):3255-3263.

［9］XU D, QU C K. Protein tyrosine phosphatases in the JAK/STAT pathway ［J］. Front Biosci J Virt Lib, 2008, 13: 4925-4932.

［10］CROKER B A, KIU H, NICHOLSON S E. SOCS regulation of the JAK/STAT signalling pathway ［J］. Semi Cell Dev Biol, 2008, 19: 414-422.

［11］SHUAI K. Regulation of cytokine signaling pathways by PIAS proteins ［J］. Cell Res, 2006, 16(2):196-202.

［12］AKIRA S. IL-6-regulated transcription factors ［J］. Int J Biochem Cell Biol, 1997, 29(12):1401-1418.

［13］WJST M, LICHTNER P, MEITINGER T, et al. STAT3 single-nucleotide polymorphisms and STAT3 mutations associated with hyper-IgE syndrome are not responsible for increased serum IgE serum levels in asthma families ［J］. Eu J Hum Genet, 2008, 17(3):352-356.

［14］HOLLAND S M, DELEO F R, ELLOUMI H Z, et al. STAT3 mutations in the hyper-IgE syndrome ［J］. New Eng J Med, 2007, 357(16):1608-1619.

［15］LITONJUA A A, TANTISIRA K G, LAKE S, et al. Polymorphisms in signal transducer and activator of transcription 3 and lung function in asthma ［J］. Respir Res, 2005, 6: 52.

［16］ZHONG Y, FENG J, CHEN B, et al. Signal transducer and activator of transcription 3 (STAT3) gene polymorphisms are associated with treatment outcomes in acute myeloid leukemia ［J］. Int J Lab Hematol, 2012, 34(4):383-389.

［17］NAIR R R, TOLENTINO J H, HAZLEHURST L A. Role of STAT3 in Transformation and Drug Resistance in CML ［J］. Front Oncol, 2012, 2: 30.

［18］TAMURA G, YIN J, WANG S, et al. E-Cadherin gene promoter hypermethylation in primary human gastric carcinomas ［J］. J Natl Cancer Ins, 2000, 92(7):569-573.

［19］DARNELL JR J E. STATs and gene regulation ［J］. Science, 1997, 277(5332):1630-1635.

［20］KIU H, NICHOLSON S. Biology and significance of the JAK/STAT signalling pathways ［J］. Growth Factors, 2012, 30(2):88-106.

［21］SEITA J, ASAKAWA M, OOEHARA J, et al. Interleukin-27 directly induces differentiation in hematopoietic stem cells ［J］. Blood, 2008, 111(4):1903-1912.

［22］HANKEY P. Regulation of hematopoietic cell development and function by Stat3 ［J］. Front Biosci, 2009, 14(1):5273-5290.

［23］KHATIB H, HUANG W, MIKHEIL D, et al. Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle ［J］. J Dairy Sci, 2009, 92(12):6186-6191.

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务川黑牛STAT3基因启动子区SNPs与生长相关性状的关联分析

Associations of Polymorphisms of Promoter Region of STAT3 Gene with Growth Traits in Wuchuan Black Cattle

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