Table 1

Sequencing results of cDNA libraries from heart tissues of Tibetan and Landrace pigs"

Table 2

Comparison analysis of filtered data from cDNA libraries of Tibetan and Landrace pig heart tissues with reference genome sequences  %"

Fig. 1

Number and length of new genes annotated in each database A. The number of new genes annotated by each database; B. The length of the new genes"

Table 3

Number of new transcripts annotated in each database"

Fig. 2

GO and KEGG annotation analysis chart of new genes A. Histogram of GO enrichment analysis of new genes: BP: virion assembly, viral life cycle, viral process, symbiont process, interspecies interaction between organisms; CC: MHC class II protein complex, MHC protein complex, myosin complex, ribosome, actin cytoskeleton, plasma membrane protein complex; MF: RNA-DNA hybrid ribonuclease activity, tumor necrosis factor receptor binding, endoribonuclease activity, producing 5′-phosphomonoesters, ATPase activity, tumor necrosis factor receptor superfamily binding. B. Bubble map of KEGG enrichment analysis of novel genes: NF-kappa B signaling pathway, coronavirus disease-COVID-19, phagosome, salivary secretion, systemic lupus erythematosus"

Fig. 3

Statistics of alternative splicing genes in Tibetan and Landrace pigs heart tissues"

Fig. 4

GO and KEGG enrichment analysis of differential alternative splicing genes A. Histogram of GO enrichment analysis of differential alternative splicing genes: BP: coenzyme metabolic process, cofactor metabolic process, rhythmic process, regulation of cell cycle G1/S phase transition, lamellipodium assembly; CC: contractile fiber, myofibril, sarcolemma, adherens junction, contractile fiber part; MF: GTPase binding, poly (A)-specific ribonuclease activity, FMN binding, actin binding, exonuclease activity, active with either ribo or deoxyribonucleic acids and producing 5′-phosphomonoesters. B. Bubble map of KEGG enrichment analysis of differential alternative splicing genes: AMPK signaling pathway, motor proteins, circadian rhythm, glucagon signaling pathway, tuberculosis"

Fig. 5

SNP mutation sites statistics A. SNP function: SILENT, NONSENSE, MISSENSE stands for synonymous mutation, meaningless mutations, misinterpretation mutation, respectively. B. SNP region: DOWNSTREAM, EXON, INTERGENIC, INTRON, SPLICE_SITE_ACCEPTOR, SPLICE_SITE_DONOR, SPLICE_SITE_REGION, TRANSCRIPT, UPSTREAM, UTR_3_PRIME, UTR_5_PRIME stands for downstream, exon, intergenic, intron, splice site acceptor, splice site donor, splice site region, transcript, upstream, 3′ untranslated region, 5′ untranslated region, respectively. C. SNP impact: HIGH, LOW, MODERATE, MODIFIER stands for high, low, moderate, no phenotypic effect itself, respectively"