富营养饮食重塑代谢性疾病易感猪关键组织mRNA剪接模式研究

doi:10.11843/j.issn.0366-6964.2022.08.012

Abstract

Abstract: The objective of this study was to investigate the effects of nutritious diet on alternative splicing (AS) and splicing factors (SFs) in key tissues of metabolic disease susceptible pigs. Twelve metabolic disease susceptible male pigs with the same health status, age and body weight were randomly divided into 2 groups. Eight pigs were fed a high fat and energy diet for 2 months as the nutrient-rich diet group (TG-HFHSD), and 4 pigs were fed a normal diet for 2 months as the control diet group (TG-CD).The adipose, liver, muscle, and hypothalamus tissues of TG-HFHSD and TG-CD were sequenced by RNA-seq, and the differential AS between the groups was identified by rMATS software, GO and KEGG enrichment analysis of differential splicing genes (DSGs), and SFs in liver was analyzed by DESeq2 software and pearson correlation coefficient. The results showed that 48 279, 39 536, 47 888 and 52 210 AS events were identified in adipose, liver, muscle, and hypothalamus, respectively, all of which were mainly of SE type, accounting for 77.09%-78.29%. The number of DSGs in adipose, liver, muscle, and hypothalamus were 528, 1 070, 570, and 600, respectively. The increased DSGs in the liver mainly came from RI and MXE with 177 and 471, respectively, while only 12-28 and 99-110 for other tissues. The enrichment of GO and KEGG showed that, in addition to the processes related to cell structure, the DSGs in liver was also significantly enriched in processes such as alternative splicing and mRNA metabolism, lipid metabolism, DNA damage and repair, complement and coagulation cascades. Fifty-six differentially expressed SFs were screened from liver, among which CLK1, PGC-1ɑ, USP4 and others were closely related to glucolipid metabolism and metabolic diseases. The results of this study showed that nutritious diet significantly increased DSGs in liver and affected the occurrence of liver metabolic diseases through splicing regulation, glucolipid metabolism and DNA damage, which will provide reference for the study of the early pathogenesis of liver metabolic diseases.

Key words: nutritious diet, metabolic fatty liver disease, metabolic disease-susceptible pigs, alternative splicing, splicing factor

CLC Number:

R589.9

XIA Boce, ZHANG Kaiyi, MIAO Jiakun, PENG Huanqi, YANG Yu, TAO Cong, WU Tianwen, WANG Yanfang, YANG Shulin. Study on the mRNA Splicing Pattern Reshaped by the Nutritious Diet in Key Tissues of Metabolic Disease Susceptible Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2537-2547.

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Study on the mRNA Splicing Pattern Reshaped by the Nutritious Diet in Key Tissues of Metabolic Disease Susceptible Pigs

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