Table 1

List of oligonucleotide primers used in this study"

Fig. 1

Restriction edonuclease analysis of recombinant vectors pET28a-ompD(A), pET-lppB(B) M1. DNA ladder (DL15000); M2. DNA ladder (DL2000); 1. Enzyme cleavage band of pET28a-ompD; 2. Enzyme cleavage band of pET28a-lppB"

Fig. 2

SDS-PAGE and Western blot analysis of rLppB, rOmpD and control GST protein M. Protein marker; 1-2. SDS-PAGE analysis of rLppB, rOmpD; 3-4. Western blot analysis of rLppB, rOmpD; 5. SDS-PAGE analysis of GST protein; 6. Western blot analysis of GST protein"

Fig. 3

Antibody from mice against rOmpD, rLppB after immunization"

Table 2

The protective efficacy of mice immunized with rOmpD, rLppB after challenge with APP serotype 1 and serotype 7"

Fig. 4

Antibody from pigs against rOmpD, rLppB after immunization"

Fig. 5

Survival of piglets after challenge with serotype 1 and serotype 7 Actincbacillus pleuropneumoniae strains A. Survival status of piglets infected with serotype 1 strain; B. Survival status of piglets infected with serotype 7 strain"

Fig. 6

Lung lesions in piglets challenged with serotypes 1 and 7 Actincbacillus pleuropneumoniae A and B are the results of challenge with serotype 1 strain, C and D are the results of challenge with serotype 7 strain. A. Severe bleeding and swelling of the lung from the control group; B. No obvious lung lesions from the survival piglets of the vaccination group; C. Severe adhesion between the lungs and the chest wall, damage to the lung surface after peeling, and severe abscess on the lung surface from the control group; D. Minor lung abscess from the survival piglets of the vaccination group"