基于智能饲喂开展哺乳母猪采食量基因组遗传评估研究

doi:10.11843/j.issn.0366-6964.2024.07.010

Abstract

Abstract:

This study aimed to analyze the genetic parameters of feed intake traits in lactating sows and conduct a genome-wide association study (GWAS) to evaluate the impact of intelligent feeding technology on these traits during lactation. Feed intake data from 922 first to fourth parity Large White pigs, born between July 2022 and May 2023, were collected to analyze feeding conditions during lactation. The Hiblup software estimated the genetic parameters of feeding traits during lactation, while Plink, GEMMA, and R software conducted a genome-wide association study to identify significant SNP loci and candidate genes associated with feed intake traits. Functional enrichment analysis was then performed on annotated genes within the 420 kb range surrounding these SNP loci. The results indicated that the heritability of feeding traits in lactating Large White pigs ranged from 0.20 to 0.27, and 28 SNP sites were significantly correlated with these traits. Annotation of the associated SNP sites identified 15 candidate genes related to lactation feeding traits, which were significantly enriched in anabolic processes including glucose, fatty acids, hormones, and coenzymes (P < 0.05). HAMP, NDUFB8, UPK1A, CHSY1, U6, and COX7A1 were identified as key candidate genes for feeding traits in Large White pigs during lactation. The above research results provide crucial genetic variation sites and associated genes linked to feeding traits in Large White pigs during lactation, establishing a foundation for further enhancement of feed intake traits by utilizing these genetic markers in future breeding programs, and also offers theoretical support for the breeding of lactating Large White sows with enhanced fertility and lactation performance.

Key words: intelligent feeding, nursing sow, feed intake breeding, genome-wide association study, SNP marker

CLC Number:

S828.2

Ruiqi ZHANG, Yanqin PANG, Zaishan LI, Xiuguo SHANG, Ganqiu LAN, Jinbiao GUO, Yunxiang ZHAO. Research on Feeding Capacity Selection of Lactating Sows Based on Intelligent Precision Feeding[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(7): 2890-2900.

Figures/Tables 6

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性状Trait	头数Number	最大值/(g·d^-1) Max	最小值/(g·d^-1) Min	平均值±标准差/(g·d^-1) Mean±Standard deviation	变异系数/% Coefficient of variation	h²
哺乳期日均采食量Average daily feed intake during lactation	902	8 407	3 508	5 695±777	13.64	0.27
哺乳期第一周日均采食量Average daily feed intake during the first week of lactation	868	6 194	947	4 065±824	20.27	0.23
哺乳期第二周日均采食量Average daily feed intake during the second week of lactation	878	8 844	2 963	6 031±956	15.85	0.26
哺乳期第三周日均采食量Average daily feed intake during the third week of lactation	742	10 461	3 438	6 543±1 064	16.26	0.20

性状Trait	哺乳期日均采食量Average daily feed intake during lactation	哺乳期第一周日均采食量Average daily feed intake during the first week of lactation	哺乳期第二周日均采食量Average daily feed intake during the second week of lactation	哺乳期第三周日均采食量Average daily feed intake during the third week of lactation	断配间隔Dismatching interval	下一胎次总产仔数Total litter size at next birth
哺乳期日均采食量Average daily feed intake during lactation	1
哺乳期第一周日均采食量Average daily feed intake during the first week of lactation	0.75^***	1
哺乳期第二周日均采食量Average daily feed intake during the second week of lactation	0.94^***	0.73^***	1
哺乳期第三周日均采食量Average daily feed intake during the third week of lactation	0.85^***	0.29^***	0.78^***	1
断配间隔Dismatching interval	-0.07	0.21	0.04	-0.27^**	1
下一胎次总产仔数Total litter size at next birth	0.18	0.14	-0.02	0.16^**	-0.87	1

性状Trait	SNP位点SNP loci	染色体Chromosome	位置Position	最小等位基因频率Minimum allele frequency	P	最近基因Nearest gene	最近距离Nearest distance
第一周日均采食量Average daily feed intake during the first week of lactation	rs321811272	6	44908270	0.147	2.83×10^-6	HAMP	120 965
	rs341818044	6	45092427	0.161	1.90×10^-6	UPK1A	-11 594
	rs330188241	6	45142409	0.161	1.90×10^-6	UPK1A	28 287
	rs3471691301	6	45223393	0.161	1.90×10^-6	U6	-99 895
	rs322027839	6	45244146	0.161	1.90×10^-6	U6	-79 142
	rs325439878	6	45811589	0.445	1.77×10^-5	COX7A1	290 876
	rs344506073	7	63617372	0.153	2.33×10^-5
	rs3473795189	7	63630576	0.155	2.84×10^-5
	rs321926366	9	123440360	0.363	7.89×10^-5
	rs81232569	17	44965892	0.188	9.31×10^-5
	rs342256202	17	44988096	0.200	6.02×10^-5
第二周日均采食量Average daily Feed intake during the second week of lactation	rs80782279	4	8954952	0.399	8.31×10^-5
	rs341143158	4	9059801	0.242	4.79×10^-5
	rs80835898	4	9059829	0.397	7.58×10^-5
	rs337240073	14	5402905	0.312	9.02×10^-5
	rs337081410	14	5402971	0.312	9.02×10^-5
	rs319708616	14	5402978	0.312	9.02×10^-5
	rs331157866	14	5736105	0.300	4.88×10^-5
	rs339438831	14	5736112	0.300	4.88×10^-5
	rs322722609	14	5736160	0.299	5.33×10^-5
第三周日均采食量Averag daily feed intake during the third week of lactation	rs327338831	1	138852678	0.454	6.14×10^-5	U6	250 527
	rs322009388	1	138949706	0.454	5.94×10^-5	U6	347 555
	rs333310113	1	139207648	0.454	5.92×10^-5
	rs338115020	1	139240890	0.454	7.03×10^-5
	rs329224371	1	139495954	0.454	7.76×10^-5	CHSY1	-234 767
	rs322312894	6	66166982	0.379	1.02×10^-4
	rs320830362	14	111845810	0.155	5.98×10^-5	NDUFB8	212 246
	rs80922182	14	116346626	0.135	9.57×10^-5	U1	-56 152

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Research on Feeding Capacity Selection of Lactating Sows Based on Intelligent Precision Feeding

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