Acta Veterinaria et Zootechnica Sinica ›› 2023, Vol. 54 ›› Issue (9): 3793-3802.doi: 10.11843/j.issn.0366-6964.2023.09.019

• PREVENTIVE VETERINARY MEDICINE • Previous Articles     Next Articles

Study on the Role of RaeR in the Regulation of Efflux Pump RaeC-RaeA-RaeB of Rimerella anatipestifer

LI Qianqian1, LIN Xin1, WEI Yilin1, CUI Haoyu1, ZOU Ronghua2, WU Xiaoni2, GE Jiazhen2, HUANG Guoliang1, ZHANG Lijuan1, ZHENG Fuying2, LIN Guozhen1*   

  1. 1. College of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, China;
    2. State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:2022-10-26 Published:2023-09-22

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Abstract: The aim of this study was to clarify the role of RaeR encoded by GE296_RS05175 gene in the regulation of efflux pump RaeC-RaeA-RaeB of Rimerella anatipestifer RA-LZ01 strain. The GE296_RS05160 gene (encoding the inner membrane protein RaeB) deletion mutant ΔRaeB and complemented strain cΔRaeB of RA-LZ01 strain were constructed. Similarly, GE296_RS05175 gene deletion mutant ΔRaeR and its complemented strain cΔRaeR were constructed. The growth curves, antimicrobial susceptibility and transcription levels of GE296_RS05160 and GE296_RS05175 genes under specific substrate stimulation were measured. Compared with the parental strain, the growth abilities of the deletion mutants had no obvious change. By comparison with the parental strain and complemented strains, the sensitivity of ΔRaeB to kanamycin, streptomycin and (sodium dodecyl sulfate, SDS) were increased, while ΔRaeR showed the opposite results. After SDS stimulation, the relative transcription levels of GE296_RS05160 gene in RA-LZ01 and ΔRaeR strains increased 2.26 and 4.64 times, respectively. The upregulation of GE296_RS05160 gene in ΔRaeR strain was significantly higher than that in RA-LZ01 strain. Under SDS stimulation, the relative transcription levels of GE296_RS05175 gene in RA-LZ01 and ΔRaeB strains decreased significantly. These results indicated that the efflux pump RaeC-RaeA-RaeB had no significant correlation with the growth of RA-LZ01 strain, but could mediate the tolerance of the strain to kanamycin, streptomycin and SDS. The transcription level of GE296_RS05175 gene reduced under SDS pressure, and RaeR,which encoded by GE296_RS05175 gene, regulated the expression of RaeB negatively.

Key words: Rimerella anatipestifer, RaeB, RaeR, regulation

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