枸杞酰胺体外对镉致成骨细胞凋亡的保护作用

doi:10.11843/j.issn.0366-6964.2023.03.038

Abstract

Abstract: This study aimed to explore the protective effect of aurantiamide acetate (AA) on cadmium (Cd)-induced apoptosis of osteoblasts. ROS1728 cells were used as osteoblast model. ROS1728 cells were pretreated with 5 μg·mL^-1 AA for 3 h, and then treated with 20 μg·mL^-1 CdCl₂ for 6 h. Flow cytometry was applied to detect cell apoptotic rate, mitochondrial membrane potential (MMP), and content of reactive oxygen species (ROS). Western blot was used to detect the expression of Bcl-2 and Bax. After Mito-tracker Red staining, High Content Analysis System (HCAS) was used to observe and analyze cellular mitochondrial texture index and structural changes. The results showed that compared with Cd treatment group, AA pretreatment significantly inhibited Cd-induced apoptosis rate increase Bax / Bcl-2 ratio rise, MMP decrease, mitochondrial texture structure index decline and ROS accumulation (P<0.01). The results showed that AA could inhibit Cd-induced apoptosis of ROS1728 cells by maintaining the integrity of mitochondrial structure via reducing the content of peroxide.

Key words: cadmium, aurantiamide acetate, ROS1728, apoptosis, mitochondrion, ROS

CLC Number:

CHANG Yingyan, ZHAO Hongyan, HU Maozhi, LIU Zongping. Protective Effect of Aurantiamide Acetate on Cd-induced Apoptosis of Osteoblasts in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1273-1280.

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Protective Effect of Aurantiamide Acetate on Cd-induced Apoptosis of Osteoblasts in vitro

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