NLRP3炎症小体及其下游炎症因子在犬乳腺肿瘤组织中的表达

doi:10.11843/j.issn.0366-6964.2022.04.025

Abstract

Abstract: The aim of this study was to investigate the expression and clinical significance of NLRP3 inflammasome and downstream inflammatory genes in canine mammary tumors(CMTs). The mRNA expression levels of NLRP3, Caspase-1, IL-1 and IL-18 in 45 CMTs (15 benign tumors and 30 malignant tumors) and 16 tumor adjacent normal mammary gland tissues were detected by real-time quantitative polymer chain reaction. The relationship between NLRP3 and clinicopathological features (tumor size, tumor subtype, histological malignant grade and prognosis) was also analyzed. The results showed that the mRNA expression of NLRP3 and downstream inflammatory factors were up-regulated in tumor tissues compared with normal mammary tissues, and the relative mRNA expression of NLRP3 (P=0.006) and Caspase-1 (P=0.048) in MCMTs was significantly higher than that in BCMTs. Besides，the expression of NLRP3 mRNA in MCMTs was statistically different in tumor type (P=0.029) and malignant grade (P=0.049). This study suggests that up-regulation of NLRP3 expression may promote canine mammary tumorigenesis, and could be a diagnostic predictor for CMTs and its malignant features.

Key words: mammary gland tumor, NLRP3, gene expression, dog

CLC Number:

LI Yan, LAN Tingying, PANG Bo, YANG Xiaonong, HUANG Jian. Expression of NLRP3 Inflammasome Signal Associated Genes in Canine Mammary Tumors and Clinical Significance[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1252-1258.

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Expression of NLRP3 Inflammasome Signal Associated Genes in Canine Mammary Tumors and Clinical Significance

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