免疫荧光检测绵羊体外受精胚胎Oct4、Cdx2表达研究

doi:10.11843/j.issn.0366-6964.2022.03.028

Abstract

Abstract: Oct4 and Cdx2 play important roles in the development of sheep pre-implantation embryos, which regulate the development of fetus and placenta. Oct4 and Cdx2 also regulate the expression of interferon-tau(IFNT), which plays a key role in embryo implantation and pregnancy recognization.In this study, embryos of different development stages were obtained by in vitro maturation,fertilization and cultured. Expression of Oct4 in the ovine embryos were analyzed using immunofluorescence staining. The results showed that expression of Oct4 protein was not detected in the zygotes and the early cleaved embryos. At the 16~32 morula cell stage, only a part of cells expressed Oct4 protein. Co-expression of Oct4 and Cdx2 was existed in the trophoblast of ovine blastocyst.

Key words: sheep, Oct4, blastocyst, trophoblast, co-expression

CLC Number:

S826.3

MAO Linjun, CHEN Ying, LEI Xi, PENG Xinrong, Qi Aladaer. Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 956-963.

References 31

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Study on Expression of Oct4 and Cdx2 in in vitro Fertilization Embryos of Sheep by Immunofluorescence Detection

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