番茄红素对壬基酚诱导小鼠脾损伤的保护作用及机制研究

doi:10.11843/j.issn.0366-6964.2022.02.030

Abstract

Abstract: The study aimed to investigate the protective effect and mechanism of lycopene (LYC) on subchronic poisoning of nonylphenol (NP) in spleen of mice. Forty ICR mice were randomly divided into four groups including control group (CON), poisoning group (NPG), lycopene control group (LCG) and lycopene intervention group (LNG). Mice in CON were given 0.1 mL corn oil at 8:30 every day, and the operation was repeated after two hours; mice in NPG were administrated 0.1 mL corn oil at 8:30 every day, and then were given 150 mg·kg^-1 NP two hours later; mice in LCG were given 5 mg·kg^-1 LYC at 8:30 every day, and then were given 0.1 mL corn oil two hours later; mice in LNG were given 5 mg·kg^-1 LYC at 8:30 every day, and then were administrated 150 mg·kg^-1 NP two hours later. After 30 days of consecutive gavage, the weight of mice was recorded, blood samples were collected from submaxillary vein, then mice were euthanized, and the spleen of mice was collected and weighed after dissection, and the difference between the groups of weight growth rate and spleen body coefficient were analyzed. The changes of immunity cytokines IL-2, IFN-γ and TNF-α in serum were examined, and the spleen oxidation and anti oxidative indexes MDA, T-AOC, CAT, GSH-Px and SOD was observed, and the protein expression in p53/Bcl-2 apoptosis pathway was detected. The results showed that compared with NPG, the weight growth rate of mice was significantly increased after LYC treatment (P<0.05), serum cytokines and spleen antioxidant enzyme activity were significantly increased (P<0.05); the expression levels of key molecules of apoptosis pathway Akt, p-Akt and Bcl-2 protein in spleen of mice after LYC treatment were significantly increased (P<0.05), and the expression level of p53 and Bax protein was significantly decreased (P<0.05). LYC has protective effect on NP induced spleen injury in mice, which was related to enhancing the anti-oxidation ability of spleen and inhibiting apoptosis of spleen cells.

Key words: lycopene, nonylphenol, oxidative stress, apoptosis

CLC Number:

S852.4

YANG Shaoqing, SHAO Chunyan, ZHOU Bin, SONG Quanjiang, WANG Xiaodu, SONG Houhui, JIANG Sheng. Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 637-645.

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Protective Effect and Mechanism of Lycopene on Spleen Injury Induced by Nonylphenol in Mice

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