PRRSV-VR2332减毒活疫苗不影响猪瘟疫苗和猪圆环病毒2型灭活疫苗诱导的抗体产生

doi:10.11843/j.issn.0366-6964.2021.011.019

Abstract

Abstract: This study intends to evaluate the interference of porcine reproductive and respiratory syndrome virus modified live attenuated virus vaccine (PRRSV MLV-vaccine) on the immune effect of PCV2 and CSFV vaccine, and analyze the effects of different immunization methods on the growth of piglets. It is expected to provide data for exploring the combined immunization of PRRSV MLV-vaccine and PCV2 and CSF vaccine. In this study, 100 piglets were selected and randomly divided into A, B, C, and D 4 groups. Group A was immunized with PCV2 vaccine 7 days after the PRRSV MLV-vaccine; group B was simultaneously immunized with PRRSV MLV-vaccine and PCV2 vaccine; group C was immunized with PCV2 vaccine and group D was immunized with PRRSV MLV-vaccine. Then another 100 piglets were selected and randomly divided into E, F, G, and H 4 groups. Group E was injected with CSFV vaccine 12 days after the PRRSV MLV-vaccine; group F was simultaneously immunized with PRRSV MLV-vaccine and CSFV vaccine; group G was immunized with CSFV vaccine and group H was immunized with PRRSV MLV-vaccine. After 4 weeks of immunization, the level of antibodies in the serum of the piglets was measured. The weight of piglets in each group was measured before and after the experiment, and the average daily gain (ADG) of piglets in different groups was calculated. In groups A-D, group A and B produced high level of antibody responses against PRRSV and PCV2. Group C only produced high level of antibody responses against PCV2, while group D produced high level of antibody responses against PRRS. Among the 4 groups of E-H, the group E and F produced high level of antibody responses against PRRSV and CSFV. Group G only produced high level of antibody responses against CSFV, while group H only produced high level of antibody responses against PRRSV. The ADG of group B among the A, B, C, and D was the highest; while the ADG of group F among the of E, F, G, and H was significantly higher than that of the other three groups. Both simultaneous immunization with PRRSV MLV-vaccine and PCV2 or CSFV vaccine, and subsequent immunization with PCV2 or CSFV vaccine after vaccination with PRRSV MLV-vaccine, could induce high levels of antibody production; in terms of humoral immunity, neither immunization nor non-immunization of the PRRSV MLV-vaccine showed significant immune interference to the other two vaccines. Therefore, “simultaneous immunization with PRRSV MLV-vaccine and PCV2 vaccine at 28 days of age, followed by immunization with CSFV vaccine 12 days later” not only induced high levels of antibody responses, but also resulted in a higher ADG.

Key words: porcine reproductive and respiratory syndrome virus modified live attenuated virus vaccine, porcine circovirus 2 vaccine, classical swine fever vaccine, immune interference, vaccine combination immunity

CLC Number:

S852.65

LI Yang, GAO Sheng, JIAO Dian, DU Xingqian, XU Lele, ZHENG Zifang, CAO Xinyu, XIAO Shuqi. PRRSV-VR2332 Modified Live Attenuated Vaccine does not Affect the Antibody Production Induced by Classical Swine Fever Vaccine and Inactivated Porcine Circovirus Type 2 Vaccine[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3175-3184.

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PRRSV-VR2332 Modified Live Attenuated Vaccine does not Affect the Antibody Production Induced by Classical Swine Fever Vaccine and Inactivated Porcine Circovirus Type 2 Vaccine

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