基于线粒体控制区的鸡不同杂交组合遗传多样性研究

doi:10.11843/j.issn.0366-6964.2021.06.008

Abstract

Abstract: The aim of this study was to investigate the genetic diversity and haplotype characteristics of the mitochondrial control region (mtDNA D-loop region) in different hybrid combinations of chickens. A total of 387 individual's mtDNA D-loop regions from 6 populations of Gushi chickens, Recessive White feather chickens and their reciprocal cross F1 generations, Tibetan chickens and F2 generations were sequenced to analyze their genetic laws and haplotype characteristics. Their maternal origins were analyzed by clustering with different red jungle fowl subspecies. The results showed that the full sequence of the D-loop region of the 6 populations was 1 231 bp, and a total of 28 polymorphic sites and one C-base deletion mutation were detected, constituting 19 haplotypes which were divided into 4 haplotype groups, A, B, C and E. Among them, the Gushi chicken and the reverse cross F1 generation were mainly A and C haplotypes, the proportions of A and C haplotypes in Gushi chicken were 53.42% and 46.58%, respectively, and the proportions of A and C haplotypes in reverse cross F1 generation were 50.75% and 49.25%, respectively. The dominant haplotype of Recessive White feather chicken, orthogonal F1 generation and F2 generation was E, accounting for 48.89%, 48.84% and 50.00%, respectively. The haplotype diversity (Hd) of the 6 chicken populations ranged from 0.496 to 0.729, and the nucleotide diversity (Pi) ranged from 0.003 40 to 0.005 41. The largest Hd and Pi values were detected in the orthogonal F1 generation, followed by the Recessive White feather chicken and the F2 generation, and the genetic diversities of the Gushi chicken and the reverse cross F1 generation populations were close to each other. Cluster analysis showed that the haplotype group A, B and Gallus gallus spadiceus were clustered in one group; haplotype group E and Gallus gallus murghi were clustered in another group; haplotype group C was clustered with 4 subspecies of red jungle fowl, including Gallus gallus murghi, Gallus gallus spadiceus, Gallus gallus gallus and Gallus gallus bankiva. The present study demonstrated that the mtDNA D-loop region followed strict matrilineal inheritance, and the genetic diversity and haplotype ratio of the progeny were basically the same as those of their female parent; the local chicken breeds in China had multiple maternal lineages, and mainly originated from Gallus gallus spadiceus.

Key words: chicken, mitochondrial control region, genetic diversity, genetic origins

CLC Number:

S831.2

TANG Xiujun, FAN Yanfeng, JIA Xiaoxu, GE Qinglian, LU Junxian, MA Lina, HAN Wei, GAO Yushi. Study on Genetic Diversity in Different Hybrid Combinations of Chickens Based on Mitochondrial Control Region[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1523-1534.

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Study on Genetic Diversity in Different Hybrid Combinations of Chickens Based on Mitochondrial Control Region

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