FTN_0109蛋白对土拉弗朗西斯菌致病力的影响

doi:10.11843/j.issn.0366-6964.2020.02.015

Abstract

Abstract: Francisella tularensis (F. tularensis) is an important zoonotic pathogen, but its virulence factors and molecular pathogenesis are still unclear. This study aimed to analyze the distribution of unknown functional protein FTN_0109 in F. tularensis, and to explore its influence on the virulence of F.tularensis. The biological function of FTN_0109 was predicted by bioinformatics softwares and its subcellular localization was detected by hypervelocity centrifugation. The FTN_0109 gene deleted strain and its trans-complemented strain were constructed by homologous recombination method. The invasion and intracellular proliferation ability of the wild-type strain and its derivates in three macrophagocytes from mice were detected, and their pathogenicity and proliferation dynamics in BALB/c mice were analyzed. The results showed that FTN_0109 was predicted to be a kind of lipoprotein, mainly distributed in the inner membrane of F. tularensis. FTN_0109 mutant strain could not proliferate in mouse macrophages and its LD₅₀ in BALB/c mice increased more than 10⁴ CFU compared with wild-type strain. The bacterial load of FTN_0109 mutant strain in infected mice showed a decreasing trend and was significantly lower than that of wild-type strain(P<0.05). The results suggested that FTN_0109 was an inner membrane lipoprotein, and its deletion could seriously reduce the survival ability of F. tularensis in macrophages and mice, and thus reduced its pathogenicity in mice.

Key words: Francisella tularensis, inner membrane protein, pathogenicity, FTN_0109

CLC Number:

S852.612

CUI Guolin, LI Bingxin, ZHANG Hanqi, WANG Jun, QI Xinyi, SU Jingliang. The Effect of the Protein FTN_0109 on the Virulence of Francisella tularensis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 337-345.

References 33

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The Effect of the Protein FTN_0109 on the Virulence of Francisella tularensis

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