N蛋白磷酸化位点突变影响猪繁殖与呼吸综合征病毒的复制及亚基因组的转录

doi:10.11843/j.issn.0366-6964.2019.12.011

Abstract

Abstract: To explore whether the N-protein phosphorylation of porcine reproductive and respiratory syndrome virus (PRRSV) affects viral replication, the ser-105 and ser-120 of the PRRSV N-protein were mutated into alanine alone or simultaneously. The rescued viruses were named as A105, A120 and A105-120, and their growth curve and subgenomic transcription level were determined respectively. The viral replication rate and titer of the mutant virus in MARC-145 were significantly lower than that of parental strain PRRSV XH-GD (P<0.01 or P<0.05). While in the primary PAM cells, the replication rate of the mutant virus decreased (P<0.01), but the final virus titer was not affected. The genomic (g) RNA transcription level of the mutant virulence group was significantly decreased (P<0.01 or P<0.05), and the phosphorylation site mutation significantly reduced the transcription of the longer subgenomic mRNA (sgmRNA)2/3 (P<0.01 or P<0.05), but increased the expression levels of the shorter sgmRNA 4/5 with time. Based on the gRNA expression level, the expression of sgmRNAs expression was analyzed. Our current work showed that the N-protein phosphorylation significantly affects the expression of sgmRNA4/5(P<0.01 or P<0.05). Mutation of N protein phosphorylation site affects PRRSV replication and subgenomic transcription.

Key words: porcine reproductive and respiratory syndrome virus, N protein, phosphorylation, subgenome

CLC Number:

S852.659.6

YI Heyou, YU Zhiqing, CHEN Yao, LI Qi, WEI Yingfang, ZHANG Guihong. Mutation of N Protein Phosphorylation Site Affects Replication and Subgenomic Transcription of Porcine Reproductive and Respiratory Syndrome Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2470-2478.

References 32

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Mutation of N Protein Phosphorylation Site Affects Replication and Subgenomic Transcription of Porcine Reproductive and Respiratory Syndrome Virus

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