北京地区猪圆环病毒3型感染的检测及基因组遗传变异分析

doi:10.11843/j.issn.0366-6964.2018.10.022

Abstract

Abstract:

A porcine circovirus type 3 (PCV3) was detected from a commercial swine farm in Beijing with an outbreak of postweaning multisystemic wasting syndrome (PMWS) in nursery pigs. This study was conducted to investigate the genetic variation of prevalent PCV3 in China and provide the clues for better control of the spread of disease, we identified its complete genome sequence. A total of 73 clinical samples from pigs with reproductive failure and respiratory disease were collected from pigs farms in Fujian province from 2016 to 2017 and these samples were used to detect PCV3 by PCR. Based on PCV3 genome sequences obtained from NCBI GenBank, three pairs of specific primers were designed and synthesized. The nucleotide sequences were amplified from eye and nasal secretions through reverse transcription-PCR (RT-PCR). The cDNA fragments were inserted into pMD19-T vector and the complete genome sequence was obtained through sequencing. Sequence analysis were run on DNAStar and DNAMAN biosoftwares. Results were as follows:The discovered PCV3 strain was named as PCV3/CN/BJ-YH2016 (GenBank accession number:MG546667). The length of its genome was 2 000 bp and the coding region includes three open reading frames (ORFs). Two of them encode replication-associated protein (Rep) and capsid protein (Cap), respectively. The homology between BJ-YH2016 and other 30 submitted PCV3 genomic sequences in GenBank is from 98.6% to 99.3%. The Rep amino acid sequence has the highest homology (100%) with an American strain PCV3-US/MO2015 (KX778720) and the lowest homology (95%) with a Chinese strain PCV3/CN/Guangdong-SG1/2016 (MF589105) where there are 16 mutations mainly located between positions 5 to 23. The Cap amino acid sequence has 100% homology with an American strain 2164. There are a few mutations comparing to other PCV3 strains but at different positions, amongst which four mutations at position 24, 26, 56, 100 comparing to PCV3-US/MO2015. In conclusion, we identified the complete PCV3 genome sequence from a sick piglet with PMWS. The genomic sequence analysis would help us to come to a better understanding on PCV3 genetic variation and its epidemiologic feature.

CLC Number:

S852.659.2

SUN Ming, MA Jun, QIAO Ming-ming, LIU Qiao-rong, TIAN Xin-sheng, YANG Xin-yan, SUN Sheng-yong, CHEN Xi-zhao. Detection and Genetic Variation Analysis of a Porcine Circovirus Type 3 in Beijing[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2261-2267.

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Detection and Genetic Variation Analysis of a Porcine Circovirus Type 3 in Beijing

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