CaN/NFAT信号通路相关基因在鸡骨骼肌中的表达规律及关联分析

doi:10.11843/j.issn.0366-6964.2017.10.005

Abstract

Abstract:

The objective of the present study was to investigate the expression profile of CaN/NFAT-dependent pathway genes, and their correlation analysis in 2 phenotypically distinct skeletal muscles were studied in chicken during the early development. qRT-PCR was used to detect the mRNA expression of CnAα, CnB1, NFATc3, MEF2C and PPARGC1A genes in lateral gastrocnemius and extensor digitorum longus of Recessive White chicken at 0,1,3,5,7 and 9 week age, the correlation among expression of these genes was analyzed. The results showed that the expression trends of all the genes between 2 phenotypically distinct skeletal muscles were similar except CnB1, and the expression level of these genes in 2 phenotypically distinct skeletal muscles was all raised from 7 to 9 week age. Comparison among the expression of these genes in 2 phenotypically distinct skeletal muscle showed that the expression level of PPARGC1A in the lateral gastrocnemius was higher than that in the extensor digitorum longus at each detected age, and the expression level of other 4 genes in the lateral gastrocnemius was higher at 3 week age and lower at 7 and 9 week age than that in the extensor digitorum longus. Correlation analysis among the 5 genes expression levels in the 2 phenotypically distinct skeletal muscles showed that there was a negative correlation between the CnAα and 2 genes (NFATc3 and PPARGC1A), and positive correlations among the expression of other genes. Overall, the expression patterns of CnAα, CnB1, NFATc3, MEF2C and PPARGC1A genes in chicken skeletal muscle are age and phenotype specific, which may be related to the regulation of the myofiber growth and type transition.

CLC Number:

S813.2

SHENG Zhong-wei, SHAN Yan-ju, SHU Jing-ting, ZHANG Ming, JI Gai-ge, TU Yun-jie, FAN Jian-hua, ZOU Jian-min. The Expression Profile of CaN/NFAT-Dependent Pathway Genes and Their Correlation Analysis in Chicken Skeletal Muscle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1825-1832.

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References

[1] 占思远, 李利, 王林杰, 等. 骨骼肌发育调控相关lncRNAs研究进展[J]. 畜牧兽医学报, 2016, 47(4): 637-644.
ZHAN S Y, LI L, WANG L J, et al. Research progress of long noncoding RNAs in the regulation of skeletal muscle development[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(4): 637-644. (in Chinese)
[2] SCHIAFFINO S, REGGIANI C. Molecular diversity of myofibrillar proteins: gene regulation and functional significance[J]. Physiol Rev, 1996, 76(2): 371-423.
[3] GARRY D J, BASSEL-DUBY R S, RICHARDSON J A, et al. Postnatal development and plasticity of specialized muscle fiber characteristics in the hindlimb[J]. Dev Genet, 1996, 19(2): 146-156.
[4] CALABRIA E, CICILIOT S, MORETTI I, et al. NFAT isoforms control activity-dependent muscle fiber type specification[J]. Proc Natl Acad Sci U S A, 2009, 106(32): 13335-13340.
[5] WU H, NAVA F J, MCKINSEY T A, et al. MEF2 responds to multiple calcium-regulated signals in the control of skeletal muscle fiber type[J]. EMBO J, 2000, 19(9): 1963-1973.
[6] SCHIAFFINO S, SANDRI M, MURGIA M. Activity-dependent signaling pathways controlling muscle diversity and plasticity[J]. Physiology (Bethesda), 2007, 22(4): 269-278.
[7] CHIN E R, OLSON E N, RICHARDSON J A, et al. A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type[J]. Genes Dev, 1998, 12(16): 2499-2509.
[8] HUDSON M B, PRICE S R. Calcineurin: a poorly understood regulator of muscle mass[J]. Int J Biochem Cell Biol, 2013, 45(10): 2173-2178.
[9] PARSONS S A, WILKINS B J, BUENO O F, et al. Altered skeletal muscle phenotypes in calcineurin Aα and Aβ gene-targeted mice[J]. Mol Cell Biol, 2003, 23(12): 4331-4343.
[10] 单艳菊, 宋迟, 束婧婷, 等. 钙调磷酸酶催化亚基A基因(PPP3CA)在不同品种鸭发育早期肌肉中的表达及其与肌纤维特性的相关性[J]. 农业生物技术学报, 2015, 23(2): 236-243.
SHAN Y J, SONG C, SHU J T, et al. Expression profile of protein phosphatase 3 catalytic A Gene(PPP3CA) mRNA in muscles of two duck breeds (Anas platyrhynchos domestica) and its relationship with myofiber traits during early development[J]. Journal of Agricultural Biotechnology, 2015, 23(2): 236-243. (in Chinese)
[11] DELLING U, TURECKOVA J, LIM H W, et al. A calcineurin-NFATc3-dependent pathway regulates skeletal muscle differentiation and slow myosin heavy-chain expression[J]. Mol Cell Biol, 2000, 20(17): 6600-6611.
[12] EDMONDSON D G, LYONS G E, MARTIN J F, et al. Mef2 gene expression marks the cardiac and skeletal muscle lineages during mouse embryogenesis[J]. Development, 1994, 120(5): 1251-1263.
[13] POTTHOFF M J, WU H, ARNOLD M A, et al. Histone deacetylase degradation and MEF2 activation promote the formation of slow-twitch myofibers[J]. J Clin Invest, 2007, 117(9): 2459-2467.
[14] POTTHOFF M J, ARNOLD M A, MCANALLY J, et al. Regulation of skeletal muscle sarcomere integrity and postnatal muscle function by Mef2c[J]. Mol Cell Biol, 2007, 27(23): 8143-8151.
[15] ANDERSON C M, HU J X, BARNES R M, et al. Myocyte enhancer factor 2C function in skeletal muscle is required for normal growth and glucose metabolism in mice[J]. Skelet Muscle, 2015, 5: 7.
[16] ROBERTS-WILSON T K, REDDY R N, BAILEY J L, et al. Calcineurin signaling and PGC-1α expression are suppressed during muscle atrophy due to diabetes[J]. Biochim Biophys Acta, 2010, 1803(8): 960-967.
[17] BASSEL-DUBY R, OLSON E. Signaling pathways in skeletal muscle remodeling[J]. Annu Rev Biochem, 2006, 75: 19-37.
[18] LIN J D, WU H, TARR P T, et al. Transcriptional co-activator PGC-1α drives the formation of slow-twitch muscle fibres[J]. Nature, 2002, 418(6899): 797-801.
[19] SHU J T, XU W J, ZHANG M, et al. Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types[J]. Genet Mol Res, 2014, 13(1): 895-905.
[20] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the method[J]. Methods, 2001, 25(4): 402-408.
[21] EIZEMA K, VAN DER WAL D E, VAN DER BURG M M, et al. Differential expression of calcineurin and SR Ca²⁺ handling proteins in equine muscle fibers during early postnatal growth[J]. J Histochem Cytochem, 2007, 55(3): 247-254.
[22] 李玥, 袁丽霞, 杨晓静, 等. 早期和后期限饲对肉鸡腓肠肌肌纤维类型和生长轴相关基因表达的影响[J]. 动物学报, 2006, 52(6): 1133-1141.
LI Y, YUAN L X, YANG X J, et al. Effects of early and late feed restriction on myofiber types and expression of growth-related genes in gastrocnemius of broiler chickens[J]. Acta Zoologica Sinica, 2006, 52(6): 1133-1141. (in Chinese)
[23] OH M, RYBKIN I I, COPELAND V, et al. Calcineurin is necessary for the maintenance but not embryonic development of slow muscle fibers[J]. Mol Cell Biol, 2005, 25(15): 6629-6638.
[24] MITCHELL P O, MILLS S T, PAVLATH G K. Calcineurin differentially regulates maintenance and growth of phenotypically distinct muscles[J]. Am J Physiol Cell Physiol, 2002, 282(5): C984-C992.
[25] LOMONOSOVA Y N, TURTIKOVA O V, SHENKMAN B S. Erratum to: reduced expression of MyHC slow isoform in rat soleus during unloading is accompanied by alterations of endogenous inhibitors of calcineurin/NFAT signaling pathway[J]. J Muscle Res Cell Motil, 2016, 37(1-2): 53.
[26] ALLEN D L, SARTORIUS C A, SYCURO L K, et al. Different pathways regulate expression of the skeletal myosin heavy chain genes[J]. J Biol Chem, 2001, 276(47): 43524-43533.
[27] SHU J T, LI H F, SHAN Y J, et al. Expression profile of IGF-I-calcineurin-NFATc3-dependent pathway genes in skeletal muscle during early development between duck breeds differing in growth rates[J]. Dev Genes Evol, 2015, 225(3): 139-148.
[28] WAN L, MA J S, XU G Y, et al. Molecular cloning, structural analysis and tissue expression of protein phosphatase 3 catalytic subunit alpha isoform (ppp3ca) gene in Tianfu goat muscle[J]. Int J Mol Sci, 2014, 15(2): 2346-2358.
[29] SCHULZ R A, YUTZEY K E. Calcineurin signaling and NFAT activation in cardiovascular and skeletal muscle development[J]. Dev Biol, 2004, 266(1): 1-16.
[30] SAKUMA K, YAMAGUCHI A. The functional role of calcineurin in hypertrophy, regeneration, and disorders of skeletal muscle[J]. J Biomed Biot, 2010, 2010: 721219.
[31] LIU J, LIANG X J, GAN Z J. Transcriptional regulatory circuits controlling muscle fiber type)switching[J]. Sci China Life Sci, 2015, 58(4): 321-327.

The Expression Profile of CaN/NFAT-Dependent Pathway Genes and Their Correlation Analysis in Chicken Skeletal Muscle

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