猪源产新德里金属β-内酰胺酶-1大肠埃希菌的感染特性研究

doi:10.11843/j.issn.0366-6964.2016.01.020

Abstract

Abstract:

One NDM-1-Producing Escherichia coli strain，designated as E120413，was isolated from a clinical pig case in China.Here we determined its transmissibility，pathogenicity and in vivo distribution in pigs.We performed kinetic bacterial distribution assays of porcine fecal samples，blood samples and organ tissues.The results demonstrated that E120413 strain can quickly infect the Close contacts of piglets from the inoculated piglets after being given 2 mL of the bacterial suspension containing 1.1×10¹⁰ CFU of E120413 strain.In these two groups，similar numbers of organisms were isolated from the fecal samples of these two groups of piglets，giving the longest persistence terms of over 11 weeks and 9 weeks，respectively.The E120413 organisms also could be isolated from the blood samples from day 2 to day 10 in the inoculated piglets and from day 4 to day 10 in the control piglets with the peaks of 3.7×10⁵and 4.3×10⁵ CFU•mL^-1 respectively，suggesting that the E120413 organisms might reach the porcine blood circulation and tissues effectively after infection.The presence of live E120413 organisms was then observed in brain，heart，liver，spleen，lung，kidney，stomach，duodenum，and mesenteric lymph nodes in both oral delivery group and control group.The stronger persistence of E120413 organisms in the mesenteric lymph nodes (≥11 weeks) may result from the environment，which was less hostile than that in any other organs of pig based on tissue specificity.But，no obvious signs of disease and serious pathological changes were observed in these two groups of pigs during the entire experimental period.In conclusion，we have shown that the NDM-1-Producing Escherichia coli E120413 strain has the stronger transmissibility and ability to cause persistent infection in pigs.

CLC Number:

S852.612

CHANG Shi-kai，ZHAO Zhan-qin，XUE Yun，LONG Ta，DING Ke，CHENG Xiang-chao. Transmissibility of New Delhi Metallo-β-lactamase-1-Producing Escherichia coli Isolate from Pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(1): 149-156.

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Transmissibility of New Delhi Metallo-β-lactamase-1-Producing Escherichia coli Isolate from Pig

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