鼠伤寒沙门菌rpoN基因缺失株构建及生物学特性研究

doi:10.11843/j.issn.0366-6964.2016.04.017

Abstract

Abstract:

The aim of this study was to understand the role of rpoN gene on biofilm formation of S.Typhimurium.Two S.Typhimurium strains with strong biofilm-forming ability were selected for construction of deletion mutants with deficiency of gene rpoN using the Red recombination system，and complemented strains were constructed by using prokaryotic expression vector.The biofilm-forming ability，and resistance to environmental stress of the wild-type，rpoN mutant and rpoN complemented strains were compared.A quantitative real-time PCR method based on csgA and bcsA genes was established to compare the expression of their biofilm components.The results showed that the biofilm formation，mainly contributed by increased expression of curli，was significantly enhanced in the rpoN gene deletion mutants when compared with that of the wild-type strains.The biofilm formation of revertants was similar to that of the wild-type strains.Furthermore，deletion of rpoN gene of S.Typhimurium strains resulted in their increased resistance to acid and alkali environment.Therefore， RpoN was identified as one of the associated sigma factors involved in biofilm formation of S.Typhimurium.These data may be helpful for elucidating the regulatory mechanism of Salmonella biofilm formation.

CLC Number:

S852.612

SU Yang-yang，HUANG Jun，WU Bai，YIN Yun-cong，LIU Zhen，CHEN Su-juan，PENG Da-xin，LIU Xiu-fan. Construction and Biological Characterization of rpoN Gene Deletion Mutants of Salmonella Typhimurium[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 771-778.

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Construction and Biological Characterization of rpoN Gene Deletion Mutants of Salmonella Typhimurium

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