连梅提取液对湿热泻痢仔猪的血清抗氧化指标、胃肠调节酶及十二指肠凋亡基因表达的影响

doi:10.11843/j.issn.0366-6964.2017.01.020

Abstract

Abstract:

In order to explore the effect of Lianmei extract on antioxidant index，gastrointestinal regulatory enzymes and duodenal apoptosis gene expression of heat and humidity diarrhea swine.Established heat and humidity environment artificially，and the swine fed E.coli to induce heat and humidity diarrhea model.The diarrhea swine were randomly divided into model group，positive drug group and Lianmei extract with high-，middle-and low dose groups，swine in the control group were fed at normal environment.Detected antioxidant enzymes，gastrointestinal regulatory enzymes，liver ATP enzymes vitality and duodenum apoptosis gene Caspase-3，-8，-9 mRNA expression changes for analysing the treatment mechanism of Lianmei extract.The results showed that the swine treated by Lianmei extract，The serum content of T-SOD and GSH-Px enzyme activity increased，LDH and MDA reduced，compared with model group，the difference was extremely significant (P<0.01).Acetylcholinesterase，amylase，liver Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase vitality significantly increased (P<0.05).Growth hormone，thyroid hormone T3 secretion improved significantly (P<0.05)，Glucocorticoids secretion decreased significantly (P<0.05).Meanwhile, duodenal apoptosis genes of swine recoveryed at different degree.Only the middle dose group Caspase-8 mRNA transcription decreased significantly to 2.62 times of that from the control (P<0.01).High- and middle dose group Caspase-3 mRNA transcription were reduced to 5.82 and 3.29 times of that from the control，compared with the model group，the difference was significant (P<0.05) or extremely significant (P<0.01).However，Caspase-9 mRNA transcription was significantly reduced (P<0.05) at high-，middle，low dose group and the amount of reduction was proportional to the concentration of the drug.The results indicated that Lianmei extract could achieve therapeutic heat and humidity diarrhea swine by enhancing diarrhea pigs anti-oxidative damage，improving gastrointestinal digestion and absorption capacity，promoting the secretion of growth hormone and reducing the expression of apoptosis gene.

CLC Number:

S853.74

ZHU Mai-xun，ZHAI Shao-qin，ZHENG Hua，ZHANG Yi-fan，CHEN Chun-lin，CAO Guo-wen. Effect of Lianmei Extract on the Serum Antioxidant Index，Gastrointestinal Regulatory Enzymes and Duodenal Apoptosis Gene Expression of Heat and Humidity Diarrhea Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 166-173.

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Effect of Lianmei Extract on the Serum Antioxidant Index，Gastrointestinal Regulatory Enzymes and Duodenal Apoptosis Gene Expression of Heat and Humidity Diarrhea Swine

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