青蒿素对鸡柔嫩艾美耳球虫第二代裂殖子微线基因mRNA转录及鸡盲肠组织结构的影响

doi:10.11843/j.issn.0366-6964.2014.05.023

Abstract

Abstract:

The aim of this study was to study the mechanism of artemisinin against E.tenella.At 120 h after inoculation by oral E.tenella sporulated oocysts，we collected the second generation merozoites of E.tenella from the chicken’s caecum in different groups (challenge control group，and artemisinin-treated group) and counted with erythrocytometer.mRNA transcription of microneme genes (EtMIC1，EtMIC2，EtMIC3，EtMIC4，EtMIC5) in second-generation merozoites were analyzed using quantitative real-time PCR.The pathological structural changes of chicken cecal tissue were observed by scanning electron microscopy.Compared with the challenge control group，the number of second generation merozoites of E.tenella was decreased by 25.37% (P<0.05) in artemisinin-treated group.Meanwhile，the transcription level of EtMIC1，EtMIC2，EtMIC3，EtMIC4，EtMIC5were decreased by 46.59% (P<0.05)，36.53% (P<0.05)，57.89% (P<0.01)，38.09% (P<0.05)，54.53% (P<0.01)，respectively.The cecal pathological structures were partially improved by artemisinin treatment.These results suggested that artemisinin may decrease the number of merozoites by inhibiting the expression levels of microneme genes in second-generation merozoites，and then result in alleviating the cecal lesion.

CLC Number:

S853.74

MO Ping-hua，MA Qing-tao，JI Xiao-xia，SONG Ping，TAO Jian-ping, LI Jin-gui. Effects of Artemisinin Treatment on Microneme Genes Transcription in Second-generation Merozoites and Pathological Changes of Caecum in Chickens Infected by Eimeria tenella[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 833-838.

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Effects of Artemisinin Treatment on Microneme Genes Transcription in Second-generation Merozoites and Pathological Changes of Caecum in Chickens Infected by Eimeria tenella

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