基因芯片技术在晋南牛种公牛选育中的应用

doi:10.11843/j.issn.0366-6964.2021.010.011

Abstract

Abstract: In order to better protect, develop and utilize Jinnan cattle and ensure the genetic diversity of Jinnan cattle, in this study, gene chip technology was used to detect Jinnan cattle population genetic characteristics and perform genetic evaluation of reserve bulls, and provide theoretical and technical support for molecular-assisted breeding and breeding of Jinnan cattle. Ten blood samples of Holstein, Heshun, Simmental, Yanbian and Limousin cattle with healthy and similar weight of ((350±20) kg) at the age of 18 months were collected, and 25 blood samples of Jinnan reserve bulls were collected, these were divided into 6 groups according to different breeds of cattle, the first 5 groups had 10 repetitions in each group, and Jinnan cattle reserve bulls had 25 repe-titions. The Illumina SNP 50K high-density cattle SNP chip was used to detect their genotypes, the genetic characteristics of Jinnan cattle was analyzed and compared, and the kinship matrix was used to calculate the kinship coefficient of Jinnan cattle reserve bulls, the genetic evaluation was performed by BLUP. The results showed that Jinnan cattle had a farther relationship with Holstein, Heshun, Simmental and Limousin cattle in genetic structure, and was closer to Yanbian cattle, and it was a Chinese local breed group. The Jinnan cattle reserve bulls were genetically evaluated, and the breeding value ranking of the genomic carcass weight variance of the cattle was obtained; the JN23 had the largest carcass weight multiple trait standard deviation, and it could be selected at the genome level as a beef breeding bull. The kinship was analyzed, the Jinnan cattle reserve bull family was classified to avoid inbreeding between groups.The genetic evaluation, inbred family analysis, traditional phenotypic selection and genetic disease detection were carried out on the reserve bulls of Jinnan cattle. Finally, the selected and remained bulls were JN07, JN23, JN05, JN08, JN02, JN13, JN19 and JN14 in Jinnan cattle, the combination of a variety of breeding methods has enhanced the accuracy of bull selection, which laid a foundation for improving the group selection and breeding of Jinnan cattle.

Key words: gene chip, Jinnan cattle, genetic structure, genetic evaluation, molecular-assisted selection

CLC Number:

WANG Honghao, REN Xiaokang, ZHANG Yi, GAO Huijiang, CHE Leijie, WANG Xi. Application of Gene Chip Technology in Jinnan Bull Breeding[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2803-2813.

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Application of Gene Chip Technology in Jinnan Bull Breeding

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