水貂犬瘟热病毒受体——信号淋巴细胞激活因子的细胞定位及组织分布

doi:10.11843/j.issn.0366-6964.2014.02.018

Abstract

Abstract:

In order to study cellular localization,tissue profile and regulation of signalling lymphocyte activation molecule （SLAM),a receptor of canine distemper virus （CDV),indirect immunofluorescence assay （IFA) was conducted to detect SLAM expression in Vero cells,IFA was also used to detect CDV-antigen in liver,lung,spleen,kidney and brain of CDV-infected minks; semiquantitive PCR and Indirect Immunohistochemical assay （IHA) was selected to detect SLAM expression in referred tissues of minks with and without Distemper.The results showed that viridis fluorescence was appeared in cell membrane and cytoplasm of Vero-SLAM cells,CDV and SLAM positive reaction was appeared in cell membrane and cytoplasm of liver,lung,spleen,kidney and brain of CDV-infected minks; In minks with early distemper lesions characterized by presence of the virus,higher numbers of SLAM-expressing cells were found in multiple tissues recognized as targets of CDV compared with those in control minks,more CDV-positive cells were observed than SLAM-expressing cells.These findings suggest that SLAM is expressed in cell membrane and cytoplasm of minks,especially in lung.Virus infection is associated with up-regulation of SLAM,and there is other receptor for CDV in minks.

CLC Number:

S855.3

HUANG Juan,WANG Cong,ZHANG Hong-liang,SHAN Hu. Cellular Localization and Immunohistochemical Demonstration of A Sort of Canine Distemper Virus Receptor Named Signalling Lymphocyte Activation Molecule in Minks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 289-294.

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Cellular Localization and Immunohistochemical Demonstration of A Sort of Canine Distemper Virus Receptor Named Signalling Lymphocyte Activation Molecule in Minks

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