牦牛FKBP6基因启动子区克隆、鉴定与分析

doi:10.11843/j.issn.0366-6964.2017.01.006

Abstract

Abstract:

The aim of this study was to understand 5′ regulatory region and promoter region features of FKBP6 gene, to provide evidence for exploring the mechanisms of differential expression of FKBP6 gene in yak and yak-cattle testicular tissue. The sequence of 5′ regulatory region of yak FKBP6 gene was obtained by cloning and sequencing, and was analyzed by bioinformatics methods. The core promoter region of FKBP6 gene was identified using Dual-luciferase assay system and the transcription factor binding sites related to spermatogenesis were predicted using bioinformatics software. The 5′ regulatory region of yak FKBP6 gene was 1 354 bp in length by cloning, sequencing and splicing, which shared the similarity of 99.71% with cattle. The 5′ regulatory region sequence of yak FKBP6 gene contained several potential promoter regions, typical CAAT-Box and CpG island, but no TATA-Box. The core promoter region of FKBP6 gene was located in －263-－167 nt of 5′ regulatory region of FKBP6 gene by luciferase activity analysis and contained transcription factor binding sites related to spermatogenesis including CAAT-Box, E-Box, CTCF and CREB. The identification of the FKBP6 gene core promoter and the transcription factor binding sites provide a basis for further research on the regulation of FKBP6 gene expression in yak testis.

CLC Number:

YAO Yi-long, LI Bo-jiang, LI Qi-fa. Cloning and Identification of Promoter of Yak FKBP6 Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 52-59.

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Cloning and Identification of Promoter of Yak FKBP6 Gene

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