绵羊多羔主效基因FecB高通量检测方法的建立及应用

doi:10.11843/j.issn.0366-6964.2017.01.005

Abstract

Abstract:

Based on the previous studies, the aim of this study was to further improve the efficiency and accuracy of genotyping. Two high-throughput genotyping methods(Taqman and SNaPshot) for FecB detection in Chinese sheep breeds had been established in the present study. The results showed that the two methods not only reduced the cost but also saved the time and increased efficiency dramatically. The two methods had potential application value in large-scale molecular breeding introducing FecB. The detection results of 23 264 sheep including 10 Chinese indigenous sheep breeds, 3 crossbred breeds and some hybridized sheep populations collected since 2003 showed that the allele B frequencies in Small Tail Han and Hu sheep were much higher than other breeds. The allele B frequency in Small Tail Han sheep was 0.432-0.833. The allele B frequency in Luxi Blackhead sheep had been improved (up to 0.432) during upgrading and selection stage, while decreased during intercross stage. These results can provide reference data for improving sheep fecundity in sheep breeding program by introducing FecB gene.

CLC Number:

S826
S813.1

LIU Qiu-yue, HU Wen-ping, HE Xiao-yun, PAN Zhang-yuan, GUO Xiao-fei, FENG Tao, CAO Gui-ling, HUANG Dong-wei, HE Jian-ning, DI Ran, CAO Xiao-han, WANG Xiang-yu, CHU Ming-xing. Establishment of High-throughput Molecular Detection Methods for Ovine High Fecundity Major Gene FecB and Their Application[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(1): 39-51.

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Establishment of High-throughput Molecular Detection Methods for Ovine High Fecundity Major Gene FecB and Their Application

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