人参皂苷Rb1对H9N2亚型猪流感病毒诱导急性肺损伤小鼠肺组织氧自由基的影响

doi:10.11843/j.issn.0366-6964.2013.09.019

Abstract

Abstract:

To study effect of ginsenoside Rb1 on oxygen free radicals in acute lung injury of mice induced by A/swine/HeBei/012/2008/swine influenza virus (H9N2 SIV), 120 six to eight weeks old BALB/c mice were randomly divided into three groups with forty in each．The mice in control group were inoculated intranaslly with an equivalent dilution of noninfectious allantoic. And that of acute lung injury group (ALI group) and Ginsenoside Rb1 group (G-Rb1 group) both were inoculated intranasally with H9N2 SIV diluted in sterile saline，and meanwhile，animals of G-Rb1 group were treated with Ginsenoside Rb1 (10 mg·kg^－1) by intraperitoneal injection continuously for up to seven days．The clinical signs and body losses were observed in eight infected mice of each group．At the same time，at the indicated time points after infection，lung histopathology was observed and the activity of T-SOD，inhibition ability of OH·, MDA and NO content of mouse lungs were detected．The results showed that mice of ALI group appeared depression，ruffled fur，feed intake reduction and weight loss post first 2 days of infection．Furthermore，pulmonary edema，hemorrhage，and a number of inflammatory cells exuding from the alveolar were observed in lungs of infected mice，however，mice organs in the control group showed no abnormality．For mice in G-Rb1 group, clinical symptoms were significantly improved, while survival time was delayed and mortality was decreased．On the 4^th, 6^th and 8^th day after infection：the inhibition ability of OH· and T-SOD activity were significantly reduced (P<0.05) in ALI group and G-Rb1 group mice compared with that of control group mice，but the indexes of G-Rb1 group were significantly higher than that of ALI group (P<0.05)；The contents of NO and MDA were increased significantly (P<0.01) in ALI group and G-Rb1 group mice compared with that of control group mice, but these contents of G-Rb1 group was significantly lower than that of ALI group (P<0.01). Results indicate that G-Rb1 could scavenge free radicals in acute lung injury lung induced by H9N2 SIV in mice，and G-Rb1 is helpful to attenuate the lung injury induced by oxygen radicals.

CLC Number:

S853.74

LIU Bao-jian, WANG Cun-lian, XU Ming-ju, WEI Dong, WANG Guo-hua, ZHANG Rui-hua, LIU Ying, XU Tong. Effect of Ginsenoside Rb1 on Oxygen Free Radicals in Acute Lung Injury of Mice Induced by H9N2 Swine Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(9): 1468-1474.

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Effect of Ginsenoside Rb1 on Oxygen Free Radicals in Acute Lung Injury of Mice Induced by H9N2 Swine Influenza Virus

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