4株猪肺炎支原体脂膜蛋白对猪肺泡上皮细胞生长抑制作用的比较

doi:10.11843/j.issn.0366-6964.2013.08.016

Abstract

Abstract:

The objective of this study was to investigate the inhibition of lipid associated membrane proteins (LAMPs) from Mycoplasma hyopneumoniae of different virulence on porcine alveolar epithelial cell lines. LAMPs were extracted from the culture of M. hyopneumoniae by Trion-X114, the suppression ratio was detected through MTT assay, and moreover the concentration of nitric oxide was measured by Griess reagent. Our results indicate that all of four kinds LAMPs can do toxic action on porcine alveolar epithelial cell lines, and the containment procedure was agreed with the virulence of M. hyopneumoniae. The inhibited effect caused by strain NJ and 168 was stronger than strain attenuate 168 and J, which the minimum IC₅₀ (0.13 mg·mL^－1) belonged to strain NJ and the maximum IC₅₀ (0.56 mg·mL^－1) belonged to strain J. Moreover, LAMPs also could induce NO. The inhibition on porcine alveolar epithelial cell lines induced by M. hyopneumoniae LAMPs is related with the virulence of Mycoplasma, while the inhibited effect of virulent strain is more powerful than attenuate strain.

CLC Number:

S852.62

NI Bo, BAI Fang-fang, WEI Yan-na, LIU Mao-jun, FENG Zhi-xin,XIONG Qi-yan, WEI Jian-zhong, SHAO Guo-qing. Comparative Analysis of the Inhibitory Effects of Lipid Associated Membrane Proteins from Four Different Strains of Mycoplasma hyopneumoniae on Porcine Alveolar Epithelial Cell Lines[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1283-1287.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/ 10.11843/j.issn.0366-6964.2013.08.016

https://www.xmsyxb.com/EN/Y2013/V44/I8/1283

References

［1］吴移谋.支原体学［M］. 2版.北京：人民卫生出版社，2008：1-59.

［2］LI Y，ZHENG H，LIU Y，et al. The complete genome sequence of Mycoplasma bovis strain Hubei-1 ［J］. PLoS One，2011，6(6)：e20999.

［3］LIU Y C，LIN I H，CHUNG W J，et al. Proteomics characterization of cytoplasmic and lipid-associated membrane proteins of human pathogen Mycoplasma fermentans M64 ［J］. PLoS One，2012，7(4)：e35304.

［4］YOU X X，ZENG Y H，WU Y M. Interactions between mycoplasma lipid-associated membrane proteins and the host cells ［J］. J Zhejiang Univ Sci B，2006，7(5)：342-350.

［5］SZCZEPANEK S M，FRASCA S Jr，SCHUMACHER V L，et al. Identification of lipoprotein MslA as a neoteric virulence factor of Mycoplasma gallisepticum ［J］. Infect Immun，2010，78(8)：3475-3483.

［6］WASHBURN L R，BIRD D W，DYBVIG K. Restoration of cytoadherence to an adherence-deficient mutant of Mycoplasma arthritidis by genetic complementation ［J］. Infect Immun，2003，71(2)：671-675.

［7］CITTI C，NOUVEL L X，BARANOWSKI E. Phase and antigenic variation in mycoplasmas ［J］. Future Microbiol，2010，5(7)：1073-1085.

［8］DAMTE D，LEE S J，HWANG M H，et al. Inflammatory responses to Mycoplasma hyopneumoniae in murine alveolar macrophage cell lines ［J］. N Z Vet J，2011，59(4)：185-190.

［9］MIYASHITA N，KAWAI Y，AKAIKE H，et al. Influence of age on the clinical different of atypical pneumonia in adults ［J］. Respirology，2012，17(7)：1073-1079.

［10］HERMEVER K，BUCHENAU I，THOMASMEYER A，et al. Chronic pneumonia in calves after experimental infection with Mycoplasma bovis strain 1067: characterization of lung pathology, persistence of variable surface protein antigens and local immune response ［J］. Acta Vet Scand，2012，54(1)：9.

［11］LI L，KRISHNAN M，BASEMAN J B，et al. Molecular cloning, expression, and characterization of a Ca²⁺-dependent, membrane-associated nuclease of Mycoplasma genitalium ［J］. J Bacteriol，2010，192(19)：4876-4884.

［12］SHIMIZU T，KIDA Y，KUWANO K. Lipid-associated membrane proteins of Mycoplasma fermentans and M. penetrans activate human immunodeficiency virus long-term repeats through Toll-like receptors ［J］. Immunology，2004，113(1)：121-129.

［13］STRAW B E， ZIMMERMAN J J， ALLAIRE S D，et al. Diseases of Swine，Ninth Edition ［M］. UK：Blackwell Publishing. 2006：701-705.

［14］曾焱华，吴移谋，余敏君,等.穿透支原体LAMPs诱导NF-κB激活介导小鼠巨噬细胞凋亡［J］. 微生物学报，2008，48(3)：355-361.

［15］ZENG Y，DENG Z，YOU X，et al. Apoptosis induced by lipid-associated membrane proteins from Mycoplasma penetrans is mediated by nuclear factor kappa B activation in mouse macrophage ［J］. Can J Microbiol，2008，54(2)：150-158.

［16］邱宏，吴移谋，游晓星，等.生殖支原体脂质相关膜蛋白激活核因子κB诱导人单核细胞表达前炎症因子及凋亡［J］.微生物学报，2007，47(5)：899-904.

［17］邓仲良，吴移谋，曾焱华，等.解脲脲原体脂质相关膜蛋白通过激活核因子κB调控诱导性一氧化氮合酶在小鼠巨噬细胞中的表达［J］.微生物学报，2006，46(5)：807-811.

［18］LOVE W，DOBBS N，TABOR L，et al. Toll-like receptor 2 (TLR 2) plays a major role in innate resistance in the lung against murine Mycoplasma ［J］. PLoS One，2010，5(5)：e10739.

［19］HE J，YOU X，ZENG Y，et al. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappa B through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway ［J］. Clin Vaccine Immunol，2009，16(12)：1750-1757.

［20］CHOI S Y，LIM J W，SHIMIZU T，et al. Reactive oxygen species mediate Jak2/Stat3 activation and IL-8 expression in pulmonary epithelial cells stimulated with lipid-associated membrane proteins from Mycoplasma pneumoniae ［J］. Inflamm Res，2012，61(5)：493-501.

［21］HWANG M H，DAMTE D，LEE J S，et al. Mycoplasma hyopneumoniae induces pro-inflammatory cytokine and nitric oxide production through NF-κ B and MAPK pathways in RAW264.7 cells ［J］. Vet Res Commun，2011，35(1)：21-34.

[1]	XIE Qingyun, XING Huixuan, YU Yanfei, YUAN Ting, XIONG Qiyan, XIONG Fuqiang, FENG Zhixin. Prokaryotic Expression, Polyclonal Antibody Preparation and Activity Identification of Helicase RuvA from Mycoplasma hyopneumoniae [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 271-281.
[2]	CHEN Qiwei, QUAN Heng, CHEN Shengli, LIU Donghui, YU Yongfeng, LI Caiyu, GONG Xiaowei, CHU Yuefeng. Functional Analysis of Biotin Uptake by the Energy-coupled Factor Transporter S Component MbBioY of Mycoplasma bovis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 282-289.
[3]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[4]	ZHONG Lemiao, LIU Binghui, WU Chunlin, WU Yijian. Preparation and Evaluation of Subunit Vaccine based on Adhesin Protein of Mycoplasma gallisepticum [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5171-5183.
[5]	WU Qi, ZHANG Yujuan, LIU Tong, XIN Jiuqing, XU Qingyuan. Research Progress of Mycoplasma bovis Adhesins and Adhesin-binding Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3206-3216.
[6]	JING Tingting, YIN Hao, HUANG Rong, LAN Shimei, LI Zhangcheng, YOU Liuchao, HAO Huafang, FU Lei, CHU Yuefeng. Prokaryotic Expression and Biological Function Analysis of Mycoplasma bovis 0580 Gene C-terminal Truncated [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2991-3001.
[7]	CHEN Yang, MENG Linchun, GUO Mengjiao, ZHANG Chengcheng, BO Zongyi, CHU Dianfeng, CAO Yongzhong, WU Yantao, ZHANG Xiaorong. Establishment and Preliminary Application of Indirect ELISA Method and HI Test for Detection of Mycoplasma Gallisepticum Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2062-2072.
[8]	WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.
[9]	XU Lifeng, JIA Chenyu, CHEN Fuzai, FANG Mengyuan, LIU Xiaodan, CHEN Jilong, LI Xunliang. Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2663-2676.
[10]	ZENG Jinhong, XU Qingyuan, SHA Weiping, WU Shuang, LI Yuan, XIN Jiuqing, WANG Xiumei. Development and Application of Monoclonal Antibodies against Mycoplasma mycoides subsp.Mycoides [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2290-2299.
[11]	YIN Dejing, WU Yan, YUE Jun, YANG Peng, CHEN Jing, WANG Hui, ZHANG Shuangxiang, WANG Kaigong, CHENG Zhentao. Construction of Eukaryotic Expression Vector of N-terminal Gene of LppA Protein of Mycoplasma Mycoides subsp.Capri and Analysis of Its Immune Efficacy in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 847-856.
[12]	LIU Lijia, WANG Yu, ZHANG Huanrong, WANG Jiabo. Whole Gene Analysis of 7 Mycoplasma synoviae Strains from Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 505-519.
[13]	YUE Yahui, XING Xiaoyong, WU Xiaochun, WEN Fengqin, ZHANG Hongyan, LONG Cuiqin, ZHANG Li, MA Haiyun, BAO Shijun. Expression of Elongation Factor Thermo Unstable of Mycoplasma synoviae WVU1853 Strain and Analysis of Its Adherence Characterization [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 520-528.
[14]	WANG Jia, HUA Lizhong, LIU Beibei, ZHANG Lei, YUAN Ting, GAN Yuan, WEI Yanna, SHAO Guoqing, FENG Zhixin, XIONG Qiyan. Isolation, Identification and Pathogenicity of a Mycoplasma hyorhinis Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3185-3193.
[15]	CHEN Shengli, HAO Huafang, JI Wenheng, CHU Yuefeng. Research Advances in Immunogenicity of Related Proteins of Animal Mycoplasmas [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1230-1237.

Comparative Analysis of the Inhibitory Effects of Lipid Associated Membrane Proteins from Four Different Strains of Mycoplasma hyopneumoniae on Porcine Alveolar Epithelial Cell Lines

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments