Abstract:

The present study was undertaken to screen antigen proteins with high specificity and sensitivity in Mycoplasma hyopneumoniae. Swine were vaccinated intramuscularly and intrapulmonary with Mycoplasma hyopneumoniae inactivated vaccine and attenuated M. hyopneumoniae 168 strain live vaccine. Serum samples were collected on 7, 15, 30 and 56 d post immunization. Antibody occurrence was detected using each and all of P97R1, P36, P46, DnaK proteins as specificity antigenic protein, respectively. And the further compare detection test was investigated. The results showed that P36 and P97R1 proteins based ELISA could detect relatively high maternal antibody levels; Antibody occurrence detected by DanK protein based ELISA was in accordance with that of P46 protein, and both had long-term high antibody titers. Compared with other proteins, mix proteins antigen based ELISA could detect high antibody titers on 56 d after immunization. Mix proteins based ELISA was satisfactory and high complicated with IDEXX antibody detection kit. But it has higher sensitivity than IDEXX antibody detection kit. And antibody occurrence detected by mixed proteins based ELISA was more in accordance with antibody occurrence regularity than IDEXX antibody detection kit. The results showed that the mix proteins based ELISA has high detection sensitivity, which indicated that this method can more accurately monitoring the infection status and animal immune status.