FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2013.06.005

Abstract

Abstract:

The objective of the study was to identify whether the combination of FSH and 17-beta-estradiol regulated the mRNA expression of Cyclin D1 and Cyclin E1 in the cultured immature boar Sertoli cells. Cultured immature boar Sertoli cells were treated with the combination of 17-beta-estradiol (10^-9 mol·L^-1) and FSH (50 ng·mL^-1) and added a variety of different signaling pathway inhibitors. And Real-time PCR was applied to detect the mRNA expression of Cyclin D1 and Cyclin E1. Compared to FSH (50 ng·mL^-1) or 17-beta-estradiol (10^-9mol·L^-1) alone, the combination of FSH and 17-beta-estradiol had no significant effect on the mRNA expression of Cyclin D1 mRNA and Cyclin E1(P>0.05). In addition, Rp-cAMP (cAMP inhibitor), Verapamil (L-type Ca²⁺ ionic channel inhibitor) or U0126 (ERK1/2 inhibitor) alone had no significant effect on the mRNA expression of Cyclin D1 and Cyclin E1 in comparison to the control group (no FSH or17-beta-estradiol) (P>0.05). However, three inhibitors could reduce the mRNA expression of Cyclin D1 and Cyclin E1 in a dose-dependent way (P<0.05, for all) when compared to the combined FSH (50 ng·mL^-1) and 17-beta-estradiol (10^-9 mol·L^-1). cAMP, Ca²⁺and ERK1/2 were involved in the effect of the combination of FSH and 17-beta-estradiol regulating the mRNA expression of Cyclin D1 and Cyclin E1.

CLC Number:

S828
S814.1

WANG Yi, ZHANG Jiao-jiao, WANG Yong, ZHANG Hui-qiong, SUN Si,WANG Xian-zhong, ZHANG Jia-hua. Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(6): 858-865.

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Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell

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