兔骨髓间充质干细胞向胰岛细胞分化的研究

Abstract

Abstract:

To explore the method for inducing rabbit bone marrow mesenchymal stem cells(BMSCs) into islet cells in vitro, BMSCs at 3rd generation were induced with dimethyl sulfoxide (DMSO) and high concentration of glucose.The morphological change of cells were observed after inducing. The islet cells were identified by Dithizone staining, the expression of insulin was detected by immunocytochemical staining. The insulin content was detected by ELISA. After induced 7 days,the cells appearred groups of cell mass. After 10 days, groups of cell mass got closely, whereas the control cells did not appeare group of cell mass. After treated with Dithizone(DTZ) and Immunocellulerchemistry (ICC), the induced cells both were positive and the control group both were negative.In ELISA tests,the insulin content of induced group were (27.82±0.43) and (27.89±0.25) mU·L^-1 at 7 and 10 days, that of the control group were (23.39±0.20) and (25.71±0.27)mU·L^-1. The difference was significant(P＜0.01) between induced and control groups. The result showed that DMSO with high concentration of glucose can induce rabbit BMSCs into islet cells in a relatively short period.

CLC Number:

HE Jun-dan, ZHANG Zhi-shuai, WANG Xin-zhuang, LV Jing-yu, XU Xiao-ting,JIANG Jin-hang, ZHAI Ming-sheng. Study of Inducing Rabbit BMSCs into Islet Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Study of Inducing Rabbit BMSCs into Islet Cells

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