Table 1

Scoring criteria for pulmonary lesion grades"

Table 2

Primers list for qRT-PCR"

Fig. 1

The expression of rFel d 1 was detected by Western blot 1.Bacteria without arabinose induction; 2.Bacteria with arabinose induction; 3.Multicolor prestained protein marker; 4.Lung tissue from mice induced by intraperitoneal injection of arabinose; 5.Lung tissue from mice without intraperitoneal injection of arabinose; M. Molecular weight of the multicolor prestained protein marker"

Fig. 2

The levels of rFel d 1-Specific IgE, IgG, IgG1, and IgG2a in mouse serum A. The levels of rFel d 1-Specific IgE in mouse serum; B. The levels of rFel d 1-Specific IgG in mouse serum; C. The levels of rFel d 1-Specific IgG1 in mouse serum; D. The levels of rFel d 1-Specific IgG2a in mouse serum; Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from the allergic group; ns. No significantly different from the allergic group"

Fig. 3

Detection of airway hyperreactivity in mice A. Airway responsiveness to increasing doses of acetylcholine chloride were determined using whole-body plethysmography; B. The area under the curve(AUC)of airway responsiveness changes. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group"

Fig. 4

Ear prick and stress-induced allergic reaction test A. Representative pictures of skin active cutaneous anaphylaxis; B. The area of dye leakage analysis by image J; C. Analysis of Evans Blue absorbance by spectrophotometry (620 nm); D. Change in body temperature after i.v. with rFel d 1;E. The area under the body temperature variation curve. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group"

Fig. 5

Histopathological examination of mouse lungs A. Lung tissue Masson staining (20×); B. Lung tissue HE staining (20×); C. Lung tissue PAS staining (black arrows indicate goblet cells, 20×); D. HE staining score; E. PAS staining score; F. Masson staining score. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group"

Fig. 6

The impact of recombinant E. coli strain EcNrFel d 1 on cytokine mRNA level in mouse lung tissue A. The mRNA level of IL-5;B. The mRNA level of IL-13;C. The mRNA level of GATA3; D. The mRNA level of RORγt; E. The mRNA level of IL-17A;F. The mRNA level of FOXP3. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group"

Fig. 7

Results of cell differential counting in mouse BALF A. Flow cytometry results of mouse BALF; B. Statistical chart of CD11c-CCR3+ eosinophils; C. Statistical chart of CD11c-CCR3- neutrophils. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group"

Fig. 8

Levels of differential T cells in splenocytes test A. Flow cytometry graph of CD4+CD25+FOXP3+ of T cell levels; B. Flow cytometry graph of CD4+IL-17A+ of T cell levels; C. Flow cytometry graph of CD4+IL-4+ and CD4+IFN-γ+ of T cell levels; D. Percentage chart of CD4+CD25+FOXP3+ of T cell levels; E. Percentage chart of CD4+IL-17A+ of T cell levels; F. Percentage chart of CD4+IL-4+ of T cell levels; G. Percentage chart of CD4+IFN-γ+ of T cell levels. Results are expressed as "$\bar{x} \pm s_{\bar{x}}$" of 6 mice per group. One-way ANOVA: *. P < 0.05, **. P < 0.01, ***. P < 0.001 different from allergic group; ns. No significantly different from allergic group; #. P < 0.05 different from EcNrFel d 1 group"