短链脂肪酸检测方法研究进展

doi:10.11843/j.issn.0366-6964.2025.08.008

Abstract

Abstract:

Short chain fatty acids (SCFAs) refer to a class of saturated fatty acids with less than 6 carbon atoms on the carbon chain, also known as volatile fatty acids. SCFAs not only regulate lipid metabolism and reduce the risk of obesity, but also closely related to the physicochemical properties and nutritional functions of milk and dairy products. In this paper, the pretreatment technology, instrumental detection technology and conventional quantitative methods for the analysis of SCFAs are comprehensively reviewed, so as to provide a theoretical reference for the establishment of detection methods for SCFAs.

Key words: short-chain fatty acids, pre-treatment, detection methods, quantitative methods

CLC Number:

LU Songcui, ZHENG Nan, WANG Jiaqi, ZHANG Yangdong. Research Progress on Short Chain Fatty Acids Detection Methods[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 3640-3649.

Figures/Tables 3

Fig. 1

Table 1

Derivatization reagents and reaction conditions for short-chain fatty acids"

样品 Sample	衍生试剂 Derivatization reagents	反应条件 Reaction conditions	检测数量 Number of detections	类型 Types	检测仪器 Detection instruments	定量限 Limits of quantification	文献 References
牛奶 Milk	氢氧化钠甲醇溶液、乙酰氯甲醇 Sodium hydroxide methanol, Acetyl chloride methanol	50 ℃/30 min皂化 90 ℃/150 min酯化 50℃/30min Saponification 90℃/150min Esterify	C4:0、C5:0、C6:0	总SCFAs	GC-MS	21.7~193.2 μg·L^-1	[40]
血清/脑脊液 Blood serum/cerebrospinal fluid	三甲基硅烷基(TMS) Trimethylsilyl	90 ℃/30 min衍生化 90℃/30min derivatization	C3:0、C4:0、isoC4:0 C5:0、isoC5:0、C6:0	总SCFAs	GC-MS	0.06~0.9 μmol·L^-1	[30]
粪便 Feces	氯甲酸苄酯 Benzyl chloroformate	离心、涡旋 Centrifugal, vortex	C2:0、C3:0、C4:0、C5:0、C6:0、isoC4:0、iso C5:0、anteisoC5:0、isoC6:0、anteisoC6:0	总SCFAs	GC-MS	0.1~5 pg	[41]
粪便 Feces	五氟溴苄 Pentafluorobenzyl bromide	60 ℃/90 min衍生化 60℃/90min derivatization	C2:0、C3:0、C4:0、isoC4:0、C5:0、isoC5:0、C6:0、isoC6:0	总SCFAs	GC-MS	0.244~0.977 μmol·L^-1	[42]
粪便 Feces	3-硝基苯肼 3-nitrophenylhydrazine	40 ℃/45 min衍生化 40℃/45min derivatization	C2:0、C3:0、C4:0、isoC4:0、C5:0、isoC5:0、C6:0、isoC6:0	总SCFAs	HPLC-UV	< 0.04 mmol·L^-1	[34]
粪便 Feces	氯甲酸丙酯 Propyl chloroformate	超声、涡旋 Ultrasonic, vortex	C2:0、C3:0、C4:0、isoC4:0、C5:0、isoC5:0、C6:0	游离 SCFAs	GC-MS	0.444~1.400 μmol·kg^-1	[44]
粪便 Feces	氯甲酸异丁酯 Isobutyl chloroformate	涡旋 vortex	C2:0、C3:0、C4:0、isoC4:0、C5:0	总SCFAs	GC-MS	/	[45]
牛奶 Milk	氯甲酸乙酯 Ethyl chloroformate	涡旋 vortex	C4:0、C6:0	游离 SCFAs	GC-MS	0.02μg·mL^-1	[46]
尿液/血浆 Urine /plasma	4-乙酰氨基-7-巯基-2, 1, 3-苯并噁二唑 4-acetamido-7-mercapto-2, 1, 3-benzoxadiazole	室温/5 min衍生化 Room temperature/5min derivatization	C2:0、C3:0、C4:0、C5:0、C6:0、isoC4:0、isoC5:0、anteisoC5:0、isoC6:0、anteisoC6:0	总SCFAs	LC-MS	< 25.20 ng·mL^-1	[47]

Table 1

Table 2

References 68

1	KOH A , DE VADDER F , KOVATCHEVA-DATCHARY P , et al. From dietary fiber to host physiology: short-chain fatty acids as key bacterial metabolites[J]. Cell, 2016, 165 (6): 1332- 1345. doi: 10.1016/j.cell.2016.05.041
2	STINSON L F , GAY M C L , KOLEVA P T , et al. Human milk from atopic mothers has lower levels of short chain fatty acids[J]. Front Immunol, 2020, 11, 1427. doi: 10.3389/fimmu.2020.01427
3	MACFARLANE S , MACFARLANE G T . Regulation of short-chain fatty acid production[J]. Proc Nutr Soc, 2003, 62 (1): 67- 72. doi: 10.1079/PNS2002207
4	NICHOLSON J K , HOLMES E , KINROSS J , et al. Host-gut microbiota metabolic interactions[J]. Science, 2012, 336 (6086): 1262- 1267. doi: 10.1126/science.1223813
5	SASAKI M , SUAINI N H A , AFGHANI J , et al. Systematic review of the association between short chain fatty acids and allergic diseases[J]. Allergy, 2024, 79 (7): 1789- 1811. doi: 10.1111/all.16065
6	RIOS-COVIAN D , GONZÁLEZ S , NOGACKA A M , et al. An overview on fecal branched short-chain fatty acids along human life and as related with body mass index: associated dietary and anthropometric factors[J]. Front Microbiol, 2020, 11, 973. doi: 10.3389/fmicb.2020.00973
7	CANI P D , VAN HUL M , LEFORT C , et al. Microbial regulation of organismal energy homeostasis[J]. Nat Metab, 2019, 1 (1): 34- 46. doi: 10.1038/s42255-018-0017-4
8	SUN M M , WU W , LIU Z J , et al. Microbiota metabolite short chain fatty acids, GPCR, and inflammatory bowel diseases[J]. J Gastroenterol, 2017, 52 (1): 1- 8. doi: 10.1007/s00535-016-1242-9
9	HOLMES Z C , SILVERMAN J D , DRESSMAN H K , et al. Short-chain fatty acid production by gut microbiota from children with obesity differs according to prebiotic choice and bacterial community composition[J]. mBio, 2020, 11 (4): e00914- 20.
10	RAHMAN M N , DIANTINI A , FATTAH M , et al. A highly sensitive, simple, and fast gas chromatography-mass spectrometry method for the quantification of serum short-chain fatty acids and their potential features in central obesity[J]. Anal Bioanal Chem, 2021, 413 (27): 6837- 6844. doi: 10.1007/s00216-021-03639-3
11	NATARAJAN N , HORI D , FLAVAHAN S , et al. Microbial short chain fatty acid metabolites lower blood pressure via endothelial G protein-coupled receptor 41[J]. Physiol Genomics, 2016, 48 (11): 826- 834. doi: 10.1152/physiolgenomics.00089.2016
12	REMELY M , AUMUELLER E , MEROLD C , et al. Effects of short chain fatty acid producing bacteria on epigenetic regulation of FFAR3 in type 2 diabetes and obesity[J]. Gene, 2014, 537 (1): 85- 92. doi: 10.1016/j.gene.2013.11.081
13	DE BENI ARRIGONI M , MARTINS C L , FACTORI M A . Lipid metabolism in the rumen[J]. Rumenology, 2016, 103- 126.
14	LIU L L Y , WU P F , GUO A W , et al. Research progress on the regulation of production traits by gastrointestinal microbiota in dairy cows[J]. Front Vet Sci, 2023, 10, 1206346. doi: 10.3389/fvets.2023.1206346
15	ASCHENBACH J R , KRISTENSEN N B , DONKIN S S , et al. Gluconeogenesis in dairy cows: the secret of making sweet milk from sour dough[J]. IUBMB Life, 2010, 62 (12): 869- 877. doi: 10.1002/iub.400
16	SHEN H , XU Z H , SHEN Z M , et al. The regulation of ruminal short-chain fatty acids on the functions of rumen barriers[J]. Front Physiol, 2019, 10, 1305. doi: 10.3389/fphys.2019.01305
17	刘振民. 乳脂及乳脂产品科学与技术[M]. 北京: 中国轻工业出版社, 2019.
	LIU Z M . Science and technology of milk fat and milk fat products[M]. Beijing: China Light Industry Press, 2019.
18	ROHDE J K , FUH M M , EVANGELAKOS I , et al. A gas chromatography mass spectrometry-based method for the quantification of short chain fatty acids[J]. Metabolites, 2022, 12 (2): 170. doi: 10.3390/metabo12020170
19	YAO L X , DAVIDSON E A , SHAIKH M W , et al. Quantitative analysis of short-chain fatty acids in human plasma and serum by GC-MS[J]. Anal Bioanal Chem, 2022, 414 (15): 4391- 4399. doi: 10.1007/s00216-021-03785-8
20	SMITH M , POLITE L , CHRISTY A , et al. An improved validated method for the determination of short-chain fatty acids in human fecal samples by gas chromatography with flame ionization detection (GC-FID)[J]. Metabolites, 2023, 13 (11): 1106. doi: 10.3390/metabo13111106
21	DEI CAS M , PARONI R , SACCARDO A , et al. A straightforward LC-MS/MS analysis to study serum profile of short and medium chain fatty acids[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2020, 1154 (1): 121982.
22	LI C , LIU Z Q , BATH C , et al. Optimised method for short-chain fatty acid profiling of bovine milk and serum[J]. Molecules, 2022, 27 (2): 436. doi: 10.3390/molecules27020436
23	CAI J , ZHANG J , TIAN Y , et al. Orthogonal comparison of GC-MS and ¹H NMR spectroscopy forshort chain fatty acid quantitation[J]. Anal Chem, 2017, 89 (15): 7900- 7906. doi: 10.1021/acs.analchem.7b00848
24	JACOBS D M , DELTIMPLE N , VAN VELZEN E , et al. ¹H NMR metabolite profiling of feces as a tool to assess the impact of nutrition on the human microbiome[J]. NMR Biomed, 2008, 21 (6): 615- 626. doi: 10.1002/nbm.1233
25	食品安全国家标准食品中脂肪酸的测定: GB 5009.168—2016[S]. 北京: 中国标准出版社, 2016.
	National Standards for Food Safety Determination of fatty acids in food[S]. Beijing: China National Standards Press, 2016. (in Chinese)
26	MULAT D G , FEILBERG A . GC/MS method for determining carbon isotope enrichment and concentration of underivatized short-chain fatty acids by direct aqueous solution injection of biogas digester samples[J]. Talanta, 2015, 143, 56- 63. doi: 10.1016/j.talanta.2015.04.065
27	RYO K , KEIKO N , NORIHISA W , et al. Butyric acid in saliva of chronic periodontitis patients induces transcription of the EBV lytic switch activator BZLF1: a pilot study[J]. In Vivo (Athens, Greece), 2020, 34 (2): 587- 594.
28	麦子盈, 覃思意, 李莎莎, 等. 生物样品中短链脂肪酸的分析方法研究进展[J]. 化学试剂, 2022, 44 (7): 1020- 1027.
	MAI Z Y , QIN S Y , LI S S , et al. Progress of analytic methods for short-chain fatty acids on biological samples[J]. Chemical Reagents, 2022, 44 (7): 1020- 1027.
29	SOARES DA SILVA BURATO J , VARGAS MEDINA D A , DE TOFFOLI A L , et al. Recent advances and trends in miniaturized sample preparation techniques[J]. J Sep Sci, 2020, 43 (1): 202- 225. doi: 10.1002/jssc.201900776
30	PAUTOVA A K , BURNAKOVA N A , BELOBORODOVA N V , et al. Simultaneous determination of aromatic, short-chain fatty and dicarboxylic acids in blood serum and cerebrospinal fluid by gas chromatography-mass spectrometry[J]. J Anal Chem, 2023, 78, 1942- 1954. doi: 10.1134/S1061934823140058
31	贾益群, 叶福媛, 王双, 等. 生物样品中短链脂肪酸的快速提取与分析方法[J]. 实验室研究与探索, 2012, 31 (7): 262- 264.
	JIA Y Q , YE F Y , WANG S , et al. Extraction and determination of short-chain fatty acids in biological samples[J]. Research and Exploration in Laboratory, 2012, 31 (7): 262- 264.
32	WU X , CHEN M , WANG F , et al. A new isopropyl esterification method for quantitative profiling of short-chain fatty acids in human and cow milk by gas chromatograph-mass spectrometer[J]. J Dairy Sci, 2024, 107 (8): 5366- 5375. doi: 10.3168/jds.2023-24320
33	DENG G , XIE L , XU S , et al. Fiber nanoarchitectonics for pre-treatments in facile detection of short-chain fatty acids in waste water and faecal samples[J]. Polymers(Basel), 2021, 13 (22): 3906.
34	WANG H Y , WANG C , GUO L X , et al. Simultaneous determination of short-chain fatty acids in human feces by HPLC with ultraviolet detection following chemical derivatization and solid-phase extraction segmental elution[J]. J Sep Sci, 2019, 42 (15): 2500- 2509. doi: 10.1002/jssc.201900249
35	FU Z , JIA Q , ZHANG H , et al. Simultaneous quantification of eleven short-chain fatty acids by derivatization and solid phase microextraction-gas chromatography tandem mass spectrometry[J]. J Chromatogr A, 2022, 1661, 462680. doi: 10.1016/j.chroma.2021.462680
36	BIANCHI F , DALL'ASTA M , DEL RIO D , et al. Development of a headspace solid-phase microextraction gas chromatography-mass spectrometric method for the determination of short-chain fatty acids from intestinal fermentation[J]. Food Chem, 2011, 129 (1): 200- 205. doi: 10.1016/j.foodchem.2011.04.022
37	FIORINI D , PACETTI D , GABBIANELLI R , et al. A salting out system for improving the efficiency of the headspace solid-phase microextraction of short and medium chain free fatty acids[J]. J Chromatogr A, 2015, 1409, 282- 287. doi: 10.1016/j.chroma.2015.07.051
38	GONZÁLEZ-CÓRDOVA A F , VALLEJO-CORDOBA B . Quantitative determination of short-chain free fatty acids in milk using solid-phase microextraction and gas chromatography[J]. J Agric Food Chem, 2001, 49 (10): 4603- 4608. doi: 10.1021/jf010108d
39	TRIVEDI N , ERICKSON H E , BALA V , et al. A concise review of liquid chromatography-mass spectrometry-based quantification methods for short chain fatty acids as endogenous biomarkers[J]. Int J Mol Sci, 2022, 23 (21): 13486. doi: 10.3390/ijms232113486
40	CHEN M , WANG F , WU X , et al. Updating the fatty acid profiles of retail bovine milk in China based on an improved GC-MS method: implications for nutrition[J]. Front Nutr, 2023, 10, 1204005. doi: 10.3389/fnut.2023.1204005
41	LI M , ZHU R , SONG X , et al. A sensitive method for the quantification of short-chain fatty acids by benzyl chloroformate derivatization combined with GC-MS[J]. Analyst, 2020, 145 (7): 2692- 2700. doi: 10.1039/D0AN00005A
42	HE L , PRODHAN M A I , YUAN F , et al. Simultaneous quantification of straight-chain and branched-chain short chain fatty acids by gas chromatography mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2018, 1092, 359- 367. doi: 10.1016/j.jchromb.2018.06.028
43	ORATA F. Derivatization reactions and reagents for gas chromatography analysis[C]//. In advanced gas chromatography-progress in agricultural, biomedical and industrial applications. 2012: 83-108.
44	张晓伟, 孙鑫, 李秀娟, 等. 衍生化-顶空固相微萃取-气相色谱法测定大鼠粪便中游离短链脂肪酸[J]. 华中农业大学学报, 2021, 40 (5): 160- 168.
	ZHANG X W , SUN X , LI X J , et al. Determination of free short-chain fatty acids in rat feces by derivatization-headspace solid-phase microextraction-gas chromatography[J]. Journal of Huazhong Agricultural University, 2021, 40 (5): 160- 168.
45	FURUHASHI T , SUGITATE K , NAKAI T , et al. Rapid profiling method for mammalian feces short chain fatty acids by GC-MS[J]. Anal Biochem, 2018, 543, 51- 54. doi: 10.1016/j.ab.2017.12.001
46	AMER B , NEBEL C , BERTRAM H C , et al. Novel method for quantification of individual free fatty acids in milk using an in-solution derivatisation approach and gas chromatography-mass spectrometry[J]. Int Dairy J, 2013, 32 (2): 199- 203. doi: 10.1016/j.idairyj.2013.05.016
47	JAOCHICO A , SANGARAJU D , SHAHIDI-LATHAM S K . A rapid derivatization based LC-MS/MS method for quantitation of short chain fatty acids in human plasma and urine[J]. Bioanalysis, 2019, 11 (8): 741- 753. doi: 10.4155/bio-2018-0241
48	ZHAO G H , NYMAN M , JÖNSSON J A . Rapid determination of short-chain fatty acids in colonic contents and faeces of humans and rats by acidified water-extraction and direct-injection gas chromatography[J]. Biomed Chromatogr, 2006, 20 (8): 674- 682. doi: 10.1002/bmc.580
49	GARCÍA-VILLALBA R , GIMÉNEZ-BASTIDA J A , GARCÍA-CONESA M T , et al. Alternative method for gas chromatography-mass spectrometry analysis of short-chain fatty acids in faecal samples[J]. J Sep Sci, 2012, 35 (15): 1906- 1913. doi: 10.1002/jssc.201101121
50	ZHANG C , FAN L , ZHAO H . Rapid detection of short-chain fatty acids in biological samples[J]. Chromatographia, 2020, 83 (2): 305- 310. doi: 10.1007/s10337-019-03824-8
51	晏慧莉, 张富新, 李延华, 等. 非衍生化-气相色谱法测定羊奶中短中链游离脂肪酸[J]. 食品科学, 2014, 35 (16): 138- 142. doi: 10.7506/spkx1002-6630-201416027
	YAN H L , ZHANG F X , LI Y H , et al. Determination of free fatty acids in goat milk by gas chromatography without derivatization[J]. Food Science, 2014, 35 (16): 138- 142. doi: 10.7506/spkx1002-6630-201416027
52	ZHENG X , QIU Y , ZHONG W , et al. A targeted metabolomic protocol for short-chain fatty acids and branched-chain amino acids[J]. Metabolomics, 2013, 9 (4): 818- 827. doi: 10.1007/s11306-013-0500-6
53	MICALIZZI G , BUZZANCA C , CHIAIA V , et al. Measurement of short-chain fatty acids in human plasma by means of fast gas chromatography-mass spectrometry[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2024, 1235, 124044. doi: 10.1016/j.jchromb.2024.124044
54	SCORTICHINI S , BOARELLI M C , SILVI S , et al. Development and validation of a GC-FID method for the analysis of short chain fatty acids in rat and human faeces and in fermentation fluids[J]. J Chromatogr B Analyt Technol Biomed Life Sci, 2020, 1143, 121972. doi: 10.1016/j.jchromb.2020.121972
55	LIEBISCH G , ECKER J , ROTH S , et al. Quantification of fecal short chain fatty acids by liquid chromatography tandem mass spectrometry-investigation of pre-analytic stability[J]. Biomolecules, 2019, 9 (4): 121. doi: 10.3390/biom9040121
56	CHEN Z , WU Y , SHRESTHA R , et al. Determination of total, free and esterified short-chain fatty acid in human serum by liquid chromatography-mass spectrometry[J]. Ann Clin Biochem, 2019, 56 (2): 190- 197. doi: 10.1177/0004563218801393
57	SONG H E , LEE H Y , KIM S J , et al. A facile profiling method of short chain fatty acids using liquid chromatography-mass spectrometry[J]. Metabolites, 2019, 9 (9): 193.
58	CALVIGIONI M , BERTOLINI A , CODINI S , et al. HPLC-MS-MS quantification of short-chain fatty acids actively secreted by probiotic strains[J]. Front Microbiol, 2023, 14, 1124144. doi: 10.3389/fmicb.2023.1124144
59	HUART J , CIRILLO A , SAINT-REMY A , et al. The faecal abundance of short chain fatty acids is increased in men with a non-dipping blood pressure profile[J]. Acta Cardiol, 2022, 77 (4): 307- 310. doi: 10.1080/00015385.2021.1901020
60	HU F , FURIHATA K , KATO Y , et al. Nondestructive quantification of organic compounds in whole milk without pretreatment by two-dimensional NMR spectroscopy[J]. J Agric Food Chem, 2007, 55 (11): 4307- 4311. doi: 10.1021/jf062803x
61	GÓMEZ-GALLEGO C , MORALES J M , MONLEÓN D , et al. Human breast milk NMR metabolomic profile across specific geographical locations and its association with the milk microbiota[J]. Nutrients, 2018, 10 (10): 1355. doi: 10.3390/nu10101355
62	O'CALLAGHAN T F , VÁZQUEZ-FRESNO R , SERRA-CAYUELA A , et al. Pasture feeding changes the bovine rumen and milk metabolome[J]. Metabolites, 2018, 8 (2): 27. doi: 10.3390/metabo8020027
63	WIKING L , L∅KKE M M , KIDMOSE U , et al. Comparison between novel and standard methods for analysis of free fatty acids in milk-including relation to rancid flavour[J]. Int Dairy J, 2017, 75, 22- 29. doi: 10.1016/j.idairyj.2017.07.001
64	PHAM U , ALVARADO L , SUESS G J , et al. Separation of short and medium-chain fatty acids using capillary electrophoresis with indirect photometric detection: Part Ⅰ: Identification of fatty acids in rat feces[J]. Electrophoresis, 2021, 42 (19): 1914- 1923. doi: 10.1002/elps.202100100
65	UKEDA H , FUJITA Y , SAWAMURA M , et al. Determination of short-chain fatty acids in raw milk using a microbial sensor and the relationship with milk quality[J]. Anal Sci, 1994, 10, 683- 685. doi: 10.2116/analsci.10.683
66	张亭妍, 王宏雁, 刘钟栋. 中短链脂肪酸结构脂的合成工艺[J]. 食品工业, 2021, 42 (4): 175- 179.
	ZHANG T Y , WANG H Y , LIU Z D . The synthesis process of middle- and short-chain triglycerides[J]. Food Industry, 2021, 42 (4): 175- 179.
67	卜子晨, 郑诗琪, 王佳, 等. 短链脂肪酸气相色谱-质谱测定方法的建立[J]. 中国食品学报, 2023, 23 (9): 261- 265.
	BU Z C , ZHENG S Q , WANG J , et al. Establishment of gas chromatography-mass spectrometry methodof a short-chain fatty acid[J]. Journal of Chinese Institute of Food Science and Technology, 2023, 23 (9): 261- 265.
68	SAHA S , DAY-WALSH P , SHEHATA E , et al. Development and validation of a LC-MS/MS technique for the analysis of short chain fatty acids in tissues and biological fluids without derivatisation using isotope labelled internal standards[J]. Molecules, 2021, 26 (21): 6444. doi: 10.3390/molecules26216444

方法 Methods	优点 Advantages	缺点 Disadvantages	文献 References
面积归一化法 Area normalization method	重复性较好，方法简便准确，进样浓度和进样量的变化对结果的影响小 The reproducibility is good, the method is simple and accurate, and the change of injection concentration and injection volume has little impact on the results	要求所有组分流出并被检测到; 误差大; 只能计算相对含量; 不宜用于微量物质的检测计算 All components are required to flow out and be detected; Large error; Only relative content can be calculated; It should not be used for the detection and calculation of trace substances	[66]
外标法 External standard method	操作简单，有标准曲线就能定量，计算时可以直接从标曲上读出含量 The operation is simple, there is a standard curve to quantify, and the content can be read directly from the curl when calculating	需要每次样品分析的前处理条件和色谱条件相同，因此容易出现较大误差 The pretreatment and chromatographic conditions need to be the same for each sample analysis, so they are prone to large errors	[67]
内标法 Internal standard method	定量的准确度和精密度较高，一定程度上可以校正试验前处理过程所带来的误差 The accuracy and precision of quantification are high, and the error caused by the pre-test treatment process can be corrected to a certain extent	对内标物的选择要求严格 The selection of internal standards is strict	[68]

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[9]	ZHANG Yue-bo, YAN Hua, WANG Li-gang, ZHAO Fu-ping, HOU Xin-hua, LIU Xin, GAO Hong-mei, ZHANG Long-chao, WANG Li-xian. RNA Editing and Its Detection Methods in Mammalian [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2299-2309.
[10]	TI Jin-feng，LI Zhi-jie，LI Xiu-li，ZHANG Min-min，ZHANG Yuan-yuan，DIAO You-xiang. Expression of NS1 Protein of Tembusu Virus and Development of Indirect ELISA Assay [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 970-977.
[11]	LIU Hua-nan, CAO Wei-jun, YANG Fan, ZHENG Hai-xue. Research Progress of Torovirus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(8): 1173-1181.

Research Progress on Short Chain Fatty Acids Detection Methods

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