Table 1

Sequence of primers used in this study"

Fig. 1

Mengshan cattle ear primary fibroblast (Scale bar: 150 μm) A. Mengshan cattle ear fibroblasts established by enzymatic digestion method; B. Mengshan cattle ear fibroblasts established by tissue block adhesion method"

Fig. 2

Passaged Mengshan cattle ear fibroblasts (Scale bar: 150 μm) A. Mengshan cattle ear fibroblasts of passage 2; B. Mengshan cattle ear fibroblasts of passage 5"

Fig. 3

Statistics of the viability of Mengshan cattle fibroblasts the error bar indicates±SD (n=3, independent experiments)"

Fig. 4

Growth curve of Mengshan cattle fibroblasts the error bar indicates±SD (n=3, independent experiments)"

Fig. 5

Schematic representation of the DOX-inducible system"

Fig. 6

Morphological changes of Mengshan cattle cells during the second stage of electroporation (Scale bar: 150 μm) A. 14 days post-electroporation, cells in 3i/LIF culture medium exhibited the morphology of stem cell colonies; B. 14 days post-electroporation, cells in NBFR culture medium lacked stem cell-like colony morphology; C. Mengshan cattle iPSCs clones on DR4 mouse fibroblast feeder layers proliferated normally; D. Mengshan cattle iPSCs on bovine fibroblast feeder layers could only be maintained for one passage; E. Mengshan cattle iPSCs stably passaged in 3i/LIF culture medium"

Fig. 7

AP staining of Mengshan cattle iPSCs in 3i / LIF medium (Scale bar: 300 μm)"

Fig. 8

Expression of pluripotency factors in Mengshan cattle iPSCs the error bar indicates±SD (n=3, independent experiments)"

Fig. 9

Immunofluorescence staining of pluripotent activity factors in Mengshan cattle iPSCs (Scale bar: 30 μm)"

Fig. 10

The maintenance ability of Mengshan cattle iPSCs in NBFR medium (Scale bar: 150 μm) A. After 1 passage of Mengshan cattle iPSCs in NBFR medium; B. After 5 passages of Mengshan cattle iPSCs in NBFR medium"

Fig. 11

Immunofluorescence staining of pluripotent activity factors in Mengshan cattle iPS cells(Scale bar: 60 μm)"