甲烷马赛球菌DZ1对小鼠血清氧化三甲胺和炎症因子、肝脏抗氧化能力及盲肠微生物区系的影响

doi:10.11843/j.issn.0366-6964.2024.10.039

Abstract

Abstract:

Methanomassiliicoccales is an indigenous group of methanogens in the mammalian gut that can use trimethylamine (TMA) to produce methane, but the mechanisms of its action on the host remain unclear. The aim of this study was to investigate the effects of Methanomethylophilus sp. DZ1 on serum trimethylamine-N-oxide (TMAO) and inflammatory factors, liver antioxidant capacity, and cecum microbiota in mice. Fourteen five-week-old male B7/6J mice (body weight 18.4±1.1g) were randomly divided into a control group (n=7) and a treatment group (n=7), single cage feeding. Each mouse in the treatment group was given 200 μL DZ1 (1.09×10⁹ cells·mL^-1) daily, and each mouse in the control group was given 200 μL sterile PBS solution daily. The experiment lasted for 4 weeks. The results showed that the body weight and feed intake of mice in the treatment group had no significant changes (P>0.05). Serum TMAO concentration and inflammatory factor levels of mice in the treatment group were significantly decreased (P < 0.05). The activities of superoxidase dismutase (SOD) in the liver of the treatment group were significantly increased (P=0.035), and the total antioxidant capacity (T-AOC) of the liver was significantly increased (P=0.039). The results of 16S rRNA gene sequencing of cecal bacteria showed no significant difference in bacterial community structure between the treatment group and control group (ANOSIM, P=0.161). The relative abundance of Verrucomicrobia in the treatment group significantly decreased (P=0.064). The relative abundances of Lawsonibacter, Ruminococcus, and Akkermansia were significantly decreased (P < 0.05). In summary, the strain DZ1 had no significant effect on the bacterial community structure of the cecum in mice, but decreased the levels of serum TMAO and inflammatory factors, and increased the total antioxidant capacity of the liver.

Key words: trimethylamine-N-oxide(TMAO), Methanomassiliicoccus, gut microbiome, inflammatory factors, antioxidant capacity

CLC Number:

S852.6

Xiaoxiu ZHAN, Pengyu LIU, Xiao'e XIANG, Shengyong MAO, Wei JIN. Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors, Liver Antioxidant Capacity and Cecum Microbiota in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4679-4689.

Figures/Tables 11

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References 44

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项目 Item	体积/质量 Volume/mass	成分 Component
缓冲液A Buffer A	50 mL	每100 mL含K₂HPO₄ 0.3 g，4 ℃保存
缓冲液B Buffer B	50 mL	每100 mL含(NH₄)₂SO₄ 0.6 g，MgSO₄·7H₂O 0.06 g，KH₂PO₄ 0.3 g， CaCl₂·2H₂O 0.06 g，NaCl 0.6 g，4 ℃保存
基础培养液Basal medium	400 mL	2 g胰蛋白胨、2 g酵母膏、5 g NaHCO₃
无细胞瘤胃液Cell-free rumen fluid	200 mL
NH₄Cl Ammonium chloride	1 g
辅酶M Coenzyme M	10 mL	每100 mL含辅酶M 0.4 g，4 ℃保存
脂肪酸溶液Fatty acid solution	50 mL
微量元素溶液Trace element solution	10 mL
还原剂Reductant	1 g	L-半胱氨酸盐酸盐
指示剂Indicator	1 mL	0.1% 刃天青1 mL(w/v)

类别 Categories	引物序列(5′→3′) Primers sequences (5′→3′)	参考文献 References
古菌Archaea	915f-AAG AAT TGG CGG GGG AGC AC	Jeyanathan等^[21]
	1386r-GCG GTG TGT GCA AGG AGC
甲烷马赛球菌Mmc	762f-GAC GAA GCC CTG GGT C	Jeyanathan等^[21]
	1099r-GAG GGT CTC GTT CGT TAT
胆碱降解菌基因cutC	389-aa-f-TTY GCI GGI TAY CAR CCN TT	Martínez-del Campo等^[22]
Choline degrading bacteria gene cutc	492-aa-r-TGN GGR TCI ACY CAI CCC AT

项目Item	对照组 Control	处理组 Treatment	标准误 SEM	P值 P-value
总古菌/lg10(copies·g^-1) Total archaea	5.50	5.90	0.31	0.536
甲烷马赛球菌/lg10(copies·g^-1) Mmc	3.81	4.67	0.22	0.048
甲烷马赛球菌占古菌比例/% The proportion of Mmc to archaea	3.85	11.73	4.16	0.083
细菌cutC/lg10(copies·g^-1) Bacterial CutC	7.06	6.96	0.05	0.394

项目 Items	对照组 Control	处理组 Treatment	标准误 SEM	P 值 P-value
OTUs	4 299.3	5 000.1	571.3	0.191
Chao指数Chao index	4 843.9	5 636.5	493.3	0.181
Shannon指数Shannon index	8.79	9.41	0.47	0.214
Simpson指数Simpson index	0.024	0.017	0.007	0.386

门水平 Phylums	对照组 Control	处理组 Treatment	标准误 SEM	P值 P-value
Firmicutes	67.61	70.73	2.24	0.277
Bacteroidetes	15.94	19.27	2.60	0.277
Verrucomicrobia	11.63	4.27	2.01	0.064
Proteobacteria	3.67	4.45	0.73	0.949
Actinobacteria	0.63	0.68	0.15	0.848
Unclassified	0.19	0.26	0.02	0.110
Deferribacteres	0.16	0.18	0.05	0.655

Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors, Liver Antioxidant Capacity and Cecum Microbiota in Mice

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门水平 Phylums	属水平 Genus	对照组 Control	处理组 Treatment	标准误 SEM	P 值 P-value
Firmicutes	Kineothrix	10.81	8.64	2.88	0.456
	Duncaniella	7.99	9.54	2.73	0.535
	Enterocloster	6.78	4.30	1.36	0.097
	Ligilactobacillus	6.30	6.28	3.19	0.456
	Limosilactobacillus	6.05	12.15	2.96	0.128
	Muribaculum	4.50	4.49	2.18	0.456
	Lactobacillus	3.96	9.33	3.26	0.710
	Eisenbergiella	3.40	2.67	1.04	0.710
	Anaerostipes	2.32	0.69	0.99	0.073
	Schaedlerella	2.24	2.06	0.87	0.710
	Acetatifactor	2.12	1.15	0.58	0.073
	Herbivorax	1.99	1.12	0.83	0.456
	Faecalimonas	1.26	1.94	0.38	0.165
	Lacrimispora	1.20	1.19	0.24	0.902
	Herbinix	1.14	0.17	0.58	0.053
	Dubosiella	1.10	1.15	1.11	0.710
	Lawsonibacter	1.07	0.57	0.23	0.026
	Extibacter	1.05	1.71	0.46	0.128
	Ruminococcus	1.05	0.33	0.36	0.038
	Roseburia	1.04	1.04	0.34	1.000
	Hungatella	1.02	0.65	0.24	0.209
	Blautia	1.00	1.12	0.18	0.456
	Anaerocolumna	0.94	2.04	1.05	0.318
	Mediterraneibacter	0.73	1.10	0.13	1.000
Verrucomicrobia	Akkermansia	11.63	4.26	3.28	0.007
Proteobacteria	Desulfovibrio	2.99	3.49	1.64	0.073
Bacteroidota	Odoribacter	0.54	1.05	0.54	0.620
	Paramuribaculum	0.80	1.07	0.42	0.902

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