基于RNA-Seq的无量山乌骨鸡肝脏组织脂代谢相关基因筛选及其表达分析

doi:10.11843/j.issn.0366-6964.2023.03.012

Abstract

Abstract: This study was conducted to screen out and verify differentially expressed genes and their regulatory pathways related to lipid metabolism by analyzing mRNA expression profiles in liver tissues of Wuliangshan Sooty chicken at different ages, and to provide theoretical basis for determining the expression characteristics of genes related to lipid metabolism in liver tissues of Wuliangshan Sooty chicken. In this study, D1 and D168 liver tissues were sequenced by RNA-Seq technique, and there were 3 biological replicates in each stage, transcriptome sequencing using the DNBSEQ platform, 10 differentially expressed genes were randomly selected for RT-qPCR validation. The differential genes related to lipid metabolism were screened by |log₂FC|≥2, Q value≤0.01 and KEGG pathways analysis. The differentially expressed genes of lipid metabolism were analyzed for GO enrichment, KEGG pathways, and protein interactions. A total of 100 female chicks were randomly divided into 5 groups with 20 chickens in each group. Two healthy hens were randomly slaughtered in each group at 5 stages (D1, D42, D84, D126, D168), and RNA was extracted from liver tissues. RT-qPCR was used to construct partial differential genes mRNA expression profiles. A total of 50 differentially expressed genes related to lipid metabolism were screened by transcriptome sequencing, among which 38 genes were up-regulated and 12 genes were down-regulated. The differentially expressed genes were mainly enriched in lipid metabolism, redox process, fatty acid biosynthesis, fatty acid metabolism, cholesterol biosynthesis and sterol biosynthesis. The differentially expressed genes were involved in steroid biosynthesis, fatty acid metabolism, glycerolipids metabolism, unsaturated fatty acid biosynthesis and PPAR signaling pathway. Key protein genes such as SCD, ACSBG2, SQLE, HSD17B7, LCAT and LPIN1 were screened out by protein interaction analysis, which were involved in the regulation of lipid metabolism through their mRNA expression levels. The 50 differentially expressed genes related to lipid metabolism were identified by transcriptome sequencing, and there are interactions among 42 differentially expressed genes. It provides a theoretical basis for further analysis of the molecular regulation of fat metabolism related genes in indigenous Chinese chicken.

Key words: Wuliangshan Sooty chicken, RNA-Seq, liver tissue, lipid metabolism, differentially expressed genes

CLC Number:

S831.2

OU Zhengmiao, ZHOU Jiawen, LIU Lili, WU Yun, CHEN Fenfen. Screening and Expression Analysis of Genes Related to Lipid Metabolism in Liver Tissue of Wuliangshan Sooty Chicken Based on RNA-Seq[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 976-988.

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Screening and Expression Analysis of Genes Related to Lipid Metabolism in Liver Tissue of Wuliangshan Sooty Chicken Based on RNA-Seq

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